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• W2050900440 abstract "Objective: We have cloned the rhesus monkey leptin receptor and examined its mRNA expression levels in the adipose tissue of monkeys to investigate the regulation of gene expression of the leptin receptor. Research Methods and Procedures: Monkey leptin receptor cDNA was cloned by reverse transcriptase-polymerase chain reaction (RT-PCR). Tissue distribution of monkey leptin receptor was examined by Northern blot analysis and RT-PCR. The mRNA levels of monkey leptin receptor in adipose tissue of normal (n=10), hyperinsulinemic obese (n = 8), and type 2 diabetic monkeys (n = 8) were measured by quantitative RT-PCR. Results: Monkey leptin receptor cDNA had at least two alternatively spliced isoforms (long and short forms). The long form of the leptin receptor mRNA was expressed relatively highly in liver, adipose tissue, hypothalamus, and choroid plexus, whereas the total leptin receptors were expressed in every tissue examined. The mRNA levels of the long form of the leptin receptor in adipose tissue were not correlated to body weight, fasting plasma insulin, plasma glucose, or plasma leptin levels. The mRNA levels of the long form of the leptin receptor were highly correlated to that of the total leptin receptor (long and short form). Discussion: The long form of leptin receptor mRNA existed in adipose tissue as well as in liver and hypothalamus, suggesting that the leptin receptor in adipose tissue may be functional in adipose tissue. The expression of the leptin receptor mRNA in adipose tissue is not affected by obesity, hyperinsulinemia, or diabetes." @default.
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• W2050900440 date "1998-09-01" @default.
• W2050900440 modified "2023-10-17" @default.
• W2050900440 title "Monkey Leptin Receptor mRNA: Sequence, Tissue Distribution, and mRNA Expression in the Adipose Tissue of Normal, Hyperinsulinemic, and Type 2 Diabetic Rhesus Monkeys" @default.
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• W2050900440 doi "https://doi.org/10.1002/j.1550-8528.1998.tb00363.x" @default.
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