Matches in SemOpenAlex for { <https://semopenalex.org/work/W2050921552> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2050921552 endingPage "61" @default.
- W2050921552 startingPage "55" @default.
- W2050921552 abstract "Lactate and ammonia are the two major waste products formed during mammalian cell growth. Accumulation of these side products can have a negative effect on cell growth, and has drawn recent attention because of their inhibitory effects on the specific product synthesis rate. Our aim is to reduce lactate formation in the cell culture by genetically manipulating of the pathway of lactate synthesis with an aim to achieve high monoclonal antibody production. We have partially disrupted the LDH-A gene by homologous recombination in hybridoma cells (ATCC-CRL-1606). The cells that received the newly introduced DNA were selected by G418, and an LDH-deficient cell was identified by a screening method based on medium color changing in 96-well plates. A variant cell, LDH-neo21, was identified through this screening method and was characterized. The specific productivity of lactate by LDH-neo21 cells was 50% lower than that of parental cells. Intracellular LDH enzyme activity was significantly reduced. The cell growth was improved both in terms of cell density and cell viability. Total cell density potentially reached 5 x 10(6) cells/mL while the parental hybridoma cells had a cell density of 3.5 x 10(6) cells/mL, which represented a 30% increase. The antibody production of LDH-neo21 cells was threefold greater than that of parental cells during 5-day batch culture. Polymerase chain reaction (PCR) results showed that at least one copy of the LDH-A gene was disrupted in the LDH-neo21 cells. The variant of the hybridoma cell exhibited a significant advantage of reduced lactate formation in the cell culture with a high concentration of glucose, which led to a higher production of monoclonal antibody. 2001 John Wiley & Sons, Inc." @default.
- W2050921552 created "2016-06-24" @default.
- W2050921552 creator A5022945869 @default.
- W2050921552 creator A5030987813 @default.
- W2050921552 creator A5044808093 @default.
- W2050921552 creator A5046476774 @default.
- W2050921552 creator A5056807501 @default.
- W2050921552 date "2000-01-01" @default.
- W2050921552 modified "2023-10-12" @default.
- W2050921552 title "Engineering of a mammalian cell line for reduction of lactate formation and high monoclonal antibody production" @default.
- W2050921552 cites W1508157641 @default.
- W2050921552 cites W1971290197 @default.
- W2050921552 cites W1971323013 @default.
- W2050921552 cites W1984660815 @default.
- W2050921552 cites W1991062831 @default.
- W2050921552 cites W2002172128 @default.
- W2050921552 cites W2009326360 @default.
- W2050921552 cites W2021351846 @default.
- W2050921552 cites W2038324009 @default.
- W2050921552 cites W2042716641 @default.
- W2050921552 cites W2051954404 @default.
- W2050921552 cites W2055685888 @default.
- W2050921552 cites W2058755882 @default.
- W2050921552 cites W2066231536 @default.
- W2050921552 cites W2070773866 @default.
- W2050921552 cites W2075636558 @default.
- W2050921552 cites W2082531364 @default.
- W2050921552 cites W2093788916 @default.
- W2050921552 cites W2120078340 @default.
- W2050921552 cites W2147790746 @default.
- W2050921552 cites W2161263785 @default.
- W2050921552 doi "https://doi.org/10.1002/1097-0290(20010105)72:1<55::aid-bit8>3.0.co;2-4" @default.
- W2050921552 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11084594" @default.
- W2050921552 hasPublicationYear "2000" @default.
- W2050921552 type Work @default.
- W2050921552 sameAs 2050921552 @default.
- W2050921552 citedByCount "148" @default.
- W2050921552 countsByYear W20509215522012 @default.
- W2050921552 countsByYear W20509215522013 @default.
- W2050921552 countsByYear W20509215522014 @default.
- W2050921552 countsByYear W20509215522015 @default.
- W2050921552 countsByYear W20509215522016 @default.
- W2050921552 countsByYear W20509215522017 @default.
- W2050921552 countsByYear W20509215522018 @default.
- W2050921552 countsByYear W20509215522019 @default.
- W2050921552 countsByYear W20509215522020 @default.
- W2050921552 countsByYear W20509215522021 @default.
- W2050921552 countsByYear W20509215522022 @default.
- W2050921552 countsByYear W20509215522023 @default.
- W2050921552 crossrefType "journal-article" @default.
- W2050921552 hasAuthorship W2050921552A5022945869 @default.
- W2050921552 hasAuthorship W2050921552A5030987813 @default.
- W2050921552 hasAuthorship W2050921552A5044808093 @default.
- W2050921552 hasAuthorship W2050921552A5046476774 @default.
- W2050921552 hasAuthorship W2050921552A5056807501 @default.
- W2050921552 hasConcept C1491633281 @default.
- W2050921552 hasConcept C153911025 @default.
- W2050921552 hasConcept C159654299 @default.
- W2050921552 hasConcept C542903549 @default.
- W2050921552 hasConcept C54355233 @default.
- W2050921552 hasConcept C55493867 @default.
- W2050921552 hasConcept C62112901 @default.
- W2050921552 hasConcept C79879829 @default.
- W2050921552 hasConcept C81885089 @default.
- W2050921552 hasConcept C86803240 @default.
- W2050921552 hasConceptScore W2050921552C1491633281 @default.
- W2050921552 hasConceptScore W2050921552C153911025 @default.
- W2050921552 hasConceptScore W2050921552C159654299 @default.
- W2050921552 hasConceptScore W2050921552C542903549 @default.
- W2050921552 hasConceptScore W2050921552C54355233 @default.
- W2050921552 hasConceptScore W2050921552C55493867 @default.
- W2050921552 hasConceptScore W2050921552C62112901 @default.
- W2050921552 hasConceptScore W2050921552C79879829 @default.
- W2050921552 hasConceptScore W2050921552C81885089 @default.
- W2050921552 hasConceptScore W2050921552C86803240 @default.
- W2050921552 hasIssue "1" @default.
- W2050921552 hasLocation W20509215521 @default.
- W2050921552 hasLocation W20509215522 @default.
- W2050921552 hasOpenAccess W2050921552 @default.
- W2050921552 hasPrimaryLocation W20509215521 @default.
- W2050921552 hasRelatedWork W1985998670 @default.
- W2050921552 hasRelatedWork W2072481810 @default.
- W2050921552 hasRelatedWork W2083693850 @default.
- W2050921552 hasRelatedWork W2143718611 @default.
- W2050921552 hasRelatedWork W2320661246 @default.
- W2050921552 hasRelatedWork W2347992620 @default.
- W2050921552 hasRelatedWork W2361888356 @default.
- W2050921552 hasRelatedWork W2419101316 @default.
- W2050921552 hasRelatedWork W2802411161 @default.
- W2050921552 hasRelatedWork W4307860292 @default.
- W2050921552 hasVolume "72" @default.
- W2050921552 isParatext "false" @default.
- W2050921552 isRetracted "false" @default.
- W2050921552 magId "2050921552" @default.
- W2050921552 workType "article" @default.