FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2050931425> ?p ?o ?g. }

• W2050931425 endingPage "994" @default.
• W2050931425 startingPage "985" @default.
• W2050931425 abstract "Development of transgenic edible plants, to be used as production, storage and delivery systems for recombinant vaccine antigens, is a promising strategy to obtain cost effective vaccines against infectious diseases, not least for use in developing countries. Therefore, we used Agrobacterium tumefaciens ‐mediated gene transfer to introduce the p24 gag gene encoding the nucleocapsid protein from HIV‐1 subtype C into the Arabidopsis thaliana plant genome. Eighteen plant lines were confirmed positive for the p24 gene by PCR; four of these lines showed an apparent homozygous phenotype when grown on selective medium and these lines also showed transcription of the p24 gene into its corresponding mRNA. The mRNA in all four cases generated the p24 protein in plants, as verified by Western blot analysis. The plants were shown to contain between 0.2 μg and 0.5 μg p24 protein per g of fresh tissue. Analysis of the localisation of the p24 protein showed that stem tissue contained the largest amount of protein, more than twice as much as leaf tissue, whereas no p24 protein was detected in roots. By using Southern blotting, we found that 4, 2–3, 2 and 1 T‐DNA insertion events took place in the four lines 1, 2, 7, and 10, respectively. The genetic insertions of line 1 were stable from the T 2 to the T 5 generation and gave rise to the p24 protein in all cases, as verified by Western blotting. In mice fed with fresh transgenic A. thaliana (line 10), anti‐gag IgG was obtained in serum after a booster injection with recombinant p37Gag. No immune response was observed after equal booster injection of untreated mice or mice fed with A. thaliana WT plants." @default.
• W2050931425 created "2016-06-24" @default.
• W2050931425 creator A5012007046 @default.
• W2050931425 creator A5019647556 @default.
• W2050931425 creator A5020418563 @default.
• W2050931425 creator A5049058428 @default.
• W2050931425 creator A5056596481 @default.
• W2050931425 creator A5057464667 @default.
• W2050931425 creator A5065532211 @default.
• W2050931425 creator A5071625584 @default.
• W2050931425 creator A5087134604 @default.
• W2050931425 date "2008-11-01" @default.
• W2050931425 modified "2023-10-12" @default.
• W2050931425 title "Feeding of mice with<i>Arabidopsis thaliana</i>expressing the HIV-1 subtype C p24 antigen gives rise to systemic immune responses" @default.
• W2050931425 cites W1546321334 @default.
• W2050931425 cites W1705041761 @default.
• W2050931425 cites W1768703625 @default.
• W2050931425 cites W1771101846 @default.
• W2050931425 cites W1964646310 @default.
• W2050931425 cites W1965501933 @default.
• W2050931425 cites W1974103061 @default.
• W2050931425 cites W1980475055 @default.
• W2050931425 cites W1986915375 @default.
• W2050931425 cites W1991667226 @default.
• W2050931425 cites W1998151345 @default.
• W2050931425 cites W1999329117 @default.
• W2050931425 cites W2003384499 @default.
• W2050931425 cites W2013327564 @default.
• W2050931425 cites W2015693740 @default.
• W2050931425 cites W2016512281 @default.
• W2050931425 cites W2026530548 @default.
• W2050931425 cites W2028685116 @default.
• W2050931425 cites W2039640733 @default.
• W2050931425 cites W2045946307 @default.
• W2050931425 cites W2046063743 @default.
• W2050931425 cites W2060513914 @default.
• W2050931425 cites W2064703363 @default.
• W2050931425 cites W2066161449 @default.
• W2050931425 cites W2068533834 @default.
• W2050931425 cites W2092368847 @default.
• W2050931425 cites W2097846333 @default.
• W2050931425 cites W2109352986 @default.
• W2050931425 cites W2111287973 @default.
• W2050931425 cites W2112218356 @default.
• W2050931425 cites W2116122104 @default.
• W2050931425 cites W2133166320 @default.
• W2050931425 cites W2139840282 @default.
• W2050931425 cites W2154987377 @default.
• W2050931425 cites W2158396895 @default.
• W2050931425 cites W2398672069 @default.
• W2050931425 cites W4248734872 @default.
• W2050931425 doi "https://doi.org/10.1111/j.1600-0463.2008.00900.x" @default.
• W2050931425 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19132995" @default.
• W2050931425 hasPublicationYear "2008" @default.
• W2050931425 type Work @default.
• W2050931425 sameAs 2050931425 @default.
• W2050931425 citedByCount "20" @default.
• W2050931425 countsByYear W20509314252012 @default.
• W2050931425 countsByYear W20509314252013 @default.
• W2050931425 countsByYear W20509314252014 @default.
• W2050931425 countsByYear W20509314252016 @default.
• W2050931425 countsByYear W20509314252018 @default.
• W2050931425 countsByYear W20509314252020 @default.
• W2050931425 countsByYear W20509314252022 @default.
• W2050931425 crossrefType "journal-article" @default.
• W2050931425 hasAuthorship W2050931425A5012007046 @default.
• W2050931425 hasAuthorship W2050931425A5019647556 @default.
• W2050931425 hasAuthorship W2050931425A5020418563 @default.
• W2050931425 hasAuthorship W2050931425A5049058428 @default.
• W2050931425 hasAuthorship W2050931425A5056596481 @default.
• W2050931425 hasAuthorship W2050931425A5057464667 @default.
• W2050931425 hasAuthorship W2050931425A5065532211 @default.
• W2050931425 hasAuthorship W2050931425A5071625584 @default.
• W2050931425 hasAuthorship W2050931425A5087134604 @default.
• W2050931425 hasBestOaLocation W20509314252 @default.
• W2050931425 hasConcept C102230213 @default.
• W2050931425 hasConcept C104317684 @default.
• W2050931425 hasConcept C105580179 @default.
• W2050931425 hasConcept C122172344 @default.
• W2050931425 hasConcept C133307689 @default.
• W2050931425 hasConcept C143065580 @default.
• W2050931425 hasConcept C147483822 @default.
• W2050931425 hasConcept C153911025 @default.
• W2050931425 hasConcept C159047783 @default.
• W2050931425 hasConcept C2775988993 @default.
• W2050931425 hasConcept C2776162087 @default.
• W2050931425 hasConcept C2776415932 @default.
• W2050931425 hasConcept C2779656422 @default.
• W2050931425 hasConcept C40767141 @default.
• W2050931425 hasConcept C54355233 @default.
• W2050931425 hasConcept C85265743 @default.
• W2050931425 hasConcept C86803240 @default.
• W2050931425 hasConcept C8891405 @default.
• W2050931425 hasConceptScore W2050931425C102230213 @default.
• W2050931425 hasConceptScore W2050931425C104317684 @default.
• W2050931425 hasConceptScore W2050931425C105580179 @default.
• W2050931425 hasConceptScore W2050931425C122172344 @default.
• W2050931425 hasConceptScore W2050931425C133307689 @default.

• next