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- W2050931882 abstract "Sequences encompassing the region between bp − 43 and + 8 of the pL and pR promoters of bacteriophage λ, as well as sequence variants of these promoters, were compared with respect to their ability to drive a promoterless cat gene in vivo. For both pL- and pR-based promoters, variants with one nonconsensus bp rather than the consensus promoter were found to be maximally active. Determination of promoter function in CSH26 and C600 revealed a marked strain dependence in the activity of some promoter variants. In response to the antibiotic coumermycin, which effects a reduction in DNA superhelical density in vivo, promoters were found to be activated, inhibited or unaffected, depending on their sequence. No simple correlation between a particular response and sequence features of a promoter has become apparent." @default.
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- W2050931882 date "1991-01-01" @default.
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- W2050931882 title "Bacteriophage lambda promoters pL and pR sequence determinants of in vivo activity and of sensitivity to the DNA gyrase inhibitor, coumermycin" @default.
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- W2050931882 doi "https://doi.org/10.1016/0378-1119(91)90003-t" @default.
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