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• W2051159093 abstract "Ubiquitin is important for the budding of many retroviruses and other enveloped viruses, but the precise role of ubiquitin in virus budding remains unclear. Here, we characterized the ubiquitination of the matrix (M) protein of a paramyxovirus, parainfluenza virus 5 (PIV5). The PIV5 M protein (but not the PIV5 nucleocapsid protein) was found to be targeted for monoubiquitination in transfected mammalian cells. Major sites of ubiquitin attachment identified by mass spectrometry analysis were lysine residues at amino acid positions 79/80, 130, and 247. The cumulative mutation of lysine residues 79, 80, and 130 to arginines led to an altered pattern of M protein ubiquitination and impaired viruslike particle (VLP) production. However, the cumulative mutation of lysine residues 79, 80, 130, and 247 to arginines restored M protein ubiquitination and VLP production, suggesting that ubiquitin is attached to alternative sites on the M protein when the primary ones have been removed. Additional lysine residues were targeted for mutagenesis based on the UbiPred algorithm. An M protein with seven lysine residues changed to arginines exhibited altered ubiquitination and poor VLP production. A recombinant virus encoding an M protein with seven lysines mutated was generated, and this virus exhibited a 6-fold-reduced maximum titer, with the defect being attributed mainly to the budding of noninfectious particles. The recombinant virus was assembly deficient, as judged by the redistribution of viral M and hemagglutinin-neuraminidase proteins in infected cells. Similar assembly defects were observed for the wild-type (wt) virus after treatment with a proteasome inhibitor. Collectively, these findings suggest that the monoubiquitination of the PIV5 M protein is important for proper virus assembly and for the budding of infectious particles." @default.
• W2051159093 created "2016-06-24" @default.
• W2051159093 creator A5027618631 @default.
• W2051159093 creator A5046692901 @default.
• W2051159093 creator A5048368288 @default.
• W2051159093 creator A5089765675 @default.
• W2051159093 date "2012-04-01" @default.
• W2051159093 modified "2023-09-25" @default.
• W2051159093 title "Role of Ubiquitin in Parainfluenza Virus 5 Particle Formation" @default.
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• W2051159093 doi "https://doi.org/10.1128/jvi.06021-11" @default.
• W2051159093 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3302527" @default.
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