FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051201019> ?p ?o ?g. }

• W2051201019 endingPage "677" @default.
• W2051201019 startingPage "666" @default.
• W2051201019 abstract "With recent methodological advances, molecular markers are increasingly used for semi-quantitative analyses of fungal communities. The aim to preserve quantitative relationships between genotypes through PCR places new demands on primers to accurately match target sites and provide short amplicons. The internal transcribed spacer (ITS) region of the ribosome encoding genes is a commonly used marker for many fungal groups. Here, we describe three new primers - fITS7, gITS7 and fITS9, which may be used to amplify the fungal ITS2 region by targeting sites in the 5.8S encoding gene. We evaluated the primers and compared their performance with the commonly used ITS1f primer by 454-sequencing of both artificially assembled templates and field samples. When the entire ITS region was amplified using the ITS1f/ITS4 primer combination, we found strong bias against species with longer amplicons. This problem could be overcome by using the new primers, which produce shorter amplicons and better preserve the quantitative composition of the template. In addition, the new primers yielded more diverse amplicon communities than the ITS1f primer." @default.
• W2051201019 created "2016-06-24" @default.
• W2051201019 creator A5010973905 @default.
• W2051201019 creator A5026313176 @default.
• W2051201019 creator A5026380886 @default.
• W2051201019 creator A5031706308 @default.
• W2051201019 creator A5047199465 @default.
• W2051201019 creator A5059566621 @default.
• W2051201019 creator A5060860999 @default.
• W2051201019 creator A5067436547 @default.
• W2051201019 creator A5074341662 @default.
• W2051201019 creator A5076400053 @default.
• W2051201019 creator A5089253035 @default.
• W2051201019 date "2012-07-27" @default.
• W2051201019 modified "2023-10-14" @default.
• W2051201019 title "New primers to amplify the fungal ITS2 region - evaluation by 454-sequencing of artificial and natural communities" @default.
• W2051201019 cites W1627725260 @default.
• W2051201019 cites W1903147462 @default.
• W2051201019 cites W1978431551 @default.
• W2051201019 cites W1980750600 @default.
• W2051201019 cites W1983476363 @default.
• W2051201019 cites W2010421806 @default.
• W2051201019 cites W2012575330 @default.
• W2051201019 cites W2016473919 @default.
• W2051201019 cites W2037269076 @default.
• W2051201019 cites W2039659654 @default.
• W2051201019 cites W2053975239 @default.
• W2051201019 cites W2072812562 @default.
• W2051201019 cites W2088170018 @default.
• W2051201019 cites W2097769208 @default.
• W2051201019 cites W2106308757 @default.
• W2051201019 cites W2110375946 @default.
• W2051201019 cites W2120644786 @default.
• W2051201019 cites W2124231729 @default.
• W2051201019 cites W2125570838 @default.
• W2051201019 cites W2128114769 @default.
• W2051201019 cites W2130889389 @default.
• W2051201019 cites W2131398649 @default.
• W2051201019 cites W2142565931 @default.
• W2051201019 cites W2162578061 @default.
• W2051201019 cites W4242587736 @default.
• W2051201019 doi "https://doi.org/10.1111/j.1574-6941.2012.01437.x" @default.
• W2051201019 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22738186" @default.
• W2051201019 hasPublicationYear "2012" @default.
• W2051201019 type Work @default.
• W2051201019 sameAs 2051201019 @default.
• W2051201019 citedByCount "1330" @default.
• W2051201019 countsByYear W20512010192012 @default.
• W2051201019 countsByYear W20512010192013 @default.
• W2051201019 countsByYear W20512010192014 @default.
• W2051201019 countsByYear W20512010192015 @default.
• W2051201019 countsByYear W20512010192016 @default.
• W2051201019 countsByYear W20512010192017 @default.
• W2051201019 countsByYear W20512010192018 @default.
• W2051201019 countsByYear W20512010192019 @default.
• W2051201019 countsByYear W20512010192020 @default.
• W2051201019 countsByYear W20512010192021 @default.
• W2051201019 countsByYear W20512010192022 @default.
• W2051201019 countsByYear W20512010192023 @default.
• W2051201019 crossrefType "journal-article" @default.
• W2051201019 hasAuthorship W2051201019A5010973905 @default.
• W2051201019 hasAuthorship W2051201019A5026313176 @default.
• W2051201019 hasAuthorship W2051201019A5026380886 @default.
• W2051201019 hasAuthorship W2051201019A5031706308 @default.
• W2051201019 hasAuthorship W2051201019A5047199465 @default.
• W2051201019 hasAuthorship W2051201019A5059566621 @default.
• W2051201019 hasAuthorship W2051201019A5060860999 @default.
• W2051201019 hasAuthorship W2051201019A5067436547 @default.
• W2051201019 hasAuthorship W2051201019A5074341662 @default.
• W2051201019 hasAuthorship W2051201019A5076400053 @default.
• W2051201019 hasAuthorship W2051201019A5089253035 @default.
• W2051201019 hasBestOaLocation W20512010191 @default.
• W2051201019 hasConcept C104317684 @default.
• W2051201019 hasConcept C155794727 @default.
• W2051201019 hasConcept C178790620 @default.
• W2051201019 hasConcept C185592680 @default.
• W2051201019 hasConcept C2777563447 @default.
• W2051201019 hasConcept C2993206177 @default.
• W2051201019 hasConcept C42062724 @default.
• W2051201019 hasConcept C49105822 @default.
• W2051201019 hasConcept C54355233 @default.
• W2051201019 hasConcept C67905577 @default.
• W2051201019 hasConcept C70721500 @default.
• W2051201019 hasConcept C8185291 @default.
• W2051201019 hasConcept C86803240 @default.
• W2051201019 hasConceptScore W2051201019C104317684 @default.
• W2051201019 hasConceptScore W2051201019C155794727 @default.
• W2051201019 hasConceptScore W2051201019C178790620 @default.
• W2051201019 hasConceptScore W2051201019C185592680 @default.
• W2051201019 hasConceptScore W2051201019C2777563447 @default.
• W2051201019 hasConceptScore W2051201019C2993206177 @default.
• W2051201019 hasConceptScore W2051201019C42062724 @default.
• W2051201019 hasConceptScore W2051201019C49105822 @default.
• W2051201019 hasConceptScore W2051201019C54355233 @default.
• W2051201019 hasConceptScore W2051201019C67905577 @default.
• W2051201019 hasConceptScore W2051201019C70721500 @default.
• W2051201019 hasConceptScore W2051201019C8185291 @default.
• W2051201019 hasConceptScore W2051201019C86803240 @default.

• next