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- W2051202084 abstract "E.coli C6 rel − met − cys − was cultured in a fully supplemented medium and in media lacking cysteine or methionine. tRNA isolated from the three cultures contained, respectively, a normal complement of modified nucleosides; a deficiency in thiolated nucleosides and a deficiency in methylated nucleosides. Both sulfur-deficient tPNA and methyl-deficient tRNA contained large amounts of N 6 -(Δ 2 -isopentenyl)adenosine and small amounts of the 2-methylthio derivative. Methyl-deficient tRNA contained, in addition a large amount of a cytokinin active, differently modified nucleoside that is believed to be a sulfur derivative of N 6 -(δ 2 -isopentenyl)adenosine. The structure of this compound is unknown. When methyl-deficient tRNA and the precursor to tRNA Tyr su 3$ A25 were enzymatically methylated invitro , methyl groups were incorporated into derivatives of isopentenyladenosine. These results indicate that the biosynthesis of the 2-methylthio derivative of isopentenyladenosine may occur in a sequential manner, i.e., thiolation of isopentenyladenosine followed by methylation." @default.
- W2051202084 created "2016-06-24" @default.
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- W2051202084 date "1975-01-01" @default.
- W2051202084 modified "2023-09-23" @default.
- W2051202084 title "Maturation of a hypermodified nucleoside in transfer RNA" @default.
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- W2051202084 doi "https://doi.org/10.1093/nar/2.5.691" @default.
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