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- W2051207633 abstract "Ion-pairing reversed-phase liquid chromatography (RPLC) was used to separate two polysulfonates, rutin nona(H-) sulfonate sodium and rutin deca(H-) sulfonate sodium, which have very similar chemical structures. The final product always contained both of them when one of the compounds was synthesized. Baseline separation was achieved on a C8-bonded silica column at ambient temperature. The eluent was acetonitrile–15 mM phosphate buffer solution containing 20 mM TBA (pH 6.0) (46:54, v/v). The calibration plot was linear in the concentration range 0.5–200 μg ml−1 for both analytes. The limits of detection (LODs; 254 nm) were 0.03 μg ml−1 for rutin nona(H-) sulfonate sodium and 0.04 μg ml−1 for rutin deca(H-) sulfonate sodium. Three batches of rutin deca(H-) sulfonate sodium were analyzed using the assay; the results showed that the analytical performance is really satisfactory." @default.
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- W2051207633 date "2004-05-01" @default.
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- W2051207633 title "Separation of rutin nona(H-) and deca(H-) sulfonate sodium by ion-pairing reversed-phase liquid chromatography" @default.
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- W2051207633 doi "https://doi.org/10.1016/j.chroma.2004.02.085" @default.
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