FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051208171> ?p ?o ?g. }

• W2051208171 endingPage "469" @default.
• W2051208171 startingPage "453" @default.
• W2051208171 abstract "Ionotropic glutamate receptor‐mediated responses were recorded from rat magnocellular basal forebrain neurones under voltage clamp from a somatically located patch‐clamp pipette. Currents were recorded from both acutely dissociated neurones and neurones maintained in culture for up to 6 weeks. Non‐NMDA and NMDA receptor‐mediated events could be distinguished pharmacologically using the selective agonists ( S )‐α‐amino‐3‐hydroxy‐5‐methyl‐isoxazolepropionic acid (AMPA), kainate and N ‐methyl‐D‐aspartate (NMDA), and antagonists 6‐cyano‐7‐nitroquinoxaline‐2,3‐dione (CNQX) and D(‐)‐2‐amino‐5‐phosphonopentanoic acid (AP5). Responses to rapid application of AMPA displayed pronounced and rapid desensitization. Responses to kainate showed no desensitization. Steady‐state EC 50 values for AMPA and kainate were 2.7 ± 0.4 μM ( n = 5 ) and 138 ± 25 μM ( n = 10 ), respectively. Cyclothiazide markedly increased current amplitude of responses to both agonists, whereas concanavalin A had no clear effect on either response. The selective AMPA receptor antagonist GYKI 53655 inhibited responses to kainate with an IC 50 of 1.2 ± 0.08 μM ( n = 5 ) at ‐70 mV. These data strongly suggest that AMPA receptors are the predominant non‐NMDA receptors expressed by basal forebrain neurones. At ‐70 mV, approximately 6 % of control current amplitude remained, at a maximally effective concentration of GYKI 53655. This residual response displayed desensitization, was insensitive to cyclothiazide and was potentiated by concanavalin A, suggesting that it was mediated by a kainate receptor. Current‐voltage relationships for non‐NMDA receptor‐mediated currents were obtained from both nucleated patches pulled from neurones in culture and from acutely dissociated neurones. With 30 μM spermine in the recording pipette, currents frequently displayed double‐rectification characteristic of non‐NMDA receptors with high Ca 2+ permeabilities. Ca 2+ permeability, relative to Na + and Cs + , was investigated using constant field theory. The measured Ca 2+ to Na + permeability coefficient ratio was 0.26‐3.6; median, 1.27 ( n = 15 ). Current flow through non‐NMDA receptors was inhibited by Ca 2+ , Cd 2+ and Co 2+ ions. At a holding potential of ‐70 mV, a maximally effective concentration of Cd 2+ (> 30 mM) reduced current amplitude by approximately 90 %, with an IC 50 of 44 μM. In six out of seven cells tested, block by Cd 2+ was voltage sensitive. Ca 2+ permeability of many of the non‐NMDA receptors expressed by magnocellular basal forebrain neurones may underlie the unusual sensitivity of cholinergic basal forebrain neurones to non‐NMDA receptor‐mediated excitotoxicity." @default.
• W2051208171 created "2016-06-24" @default.
• W2051208171 creator A5017799110 @default.
• W2051208171 creator A5066399408 @default.
• W2051208171 date "1998-04-01" @default.
• W2051208171 modified "2023-10-15" @default.
• W2051208171 title "Ca²⁺-permeable non-NMDA glutamate receptors in rat magnocellular basal forebrain neurones" @default.
• W2051208171 cites W135057512 @default.
• W2051208171 cites W1968333334 @default.
• W2051208171 cites W1972363392 @default.
• W2051208171 cites W1977823998 @default.
• W2051208171 cites W1978647995 @default.
• W2051208171 cites W1982978277 @default.
• W2051208171 cites W1988844930 @default.
• W2051208171 cites W1994672221 @default.
• W2051208171 cites W1996412620 @default.
• W2051208171 cites W1999076238 @default.
• W2051208171 cites W2002302770 @default.
• W2051208171 cites W2003184890 @default.
• W2051208171 cites W2004713321 @default.
• W2051208171 cites W2009561518 @default.
• W2051208171 cites W2012377202 @default.
• W2051208171 cites W2012533002 @default.
• W2051208171 cites W2020931572 @default.
• W2051208171 cites W2022420247 @default.
• W2051208171 cites W2023130009 @default.
• W2051208171 cites W2027009747 @default.
• W2051208171 cites W2047835829 @default.
• W2051208171 cites W2051084778 @default.
• W2051208171 cites W2051314670 @default.
• W2051208171 cites W2052450483 @default.
• W2051208171 cites W2054665431 @default.
• W2051208171 cites W2055320926 @default.
• W2051208171 cites W2064635896 @default.
• W2051208171 cites W2067640311 @default.
• W2051208171 cites W2072548447 @default.
• W2051208171 cites W2073201555 @default.
• W2051208171 cites W2074838365 @default.
• W2051208171 cites W2085402579 @default.
• W2051208171 cites W2091837097 @default.
• W2051208171 cites W2093374391 @default.
• W2051208171 cites W2105752803 @default.
• W2051208171 cites W2106016755 @default.
• W2051208171 cites W2106415123 @default.
• W2051208171 cites W2110534885 @default.
• W2051208171 cites W2123146696 @default.
• W2051208171 cites W2129108032 @default.
• W2051208171 cites W2148999943 @default.
• W2051208171 cites W2165662569 @default.
• W2051208171 cites W2166419272 @default.
• W2051208171 cites W2228069517 @default.
• W2051208171 cites W2273738402 @default.
• W2051208171 cites W2410384468 @default.
• W2051208171 doi "https://doi.org/10.1111/j.1469-7793.1998.453bq.x" @default.
• W2051208171 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2230879" @default.
• W2051208171 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9508809" @default.
• W2051208171 hasPublicationYear "1998" @default.
• W2051208171 type Work @default.
• W2051208171 sameAs 2051208171 @default.
• W2051208171 citedByCount "19" @default.
• W2051208171 countsByYear W20512081712013 @default.
• W2051208171 countsByYear W20512081712014 @default.
• W2051208171 countsByYear W20512081712015 @default.
• W2051208171 crossrefType "journal-article" @default.
• W2051208171 hasAuthorship W2051208171A5017799110 @default.
• W2051208171 hasAuthorship W2051208171A5066399408 @default.
• W2051208171 hasBestOaLocation W20512081711 @default.
• W2051208171 hasConcept C12554922 @default.
• W2051208171 hasConcept C126322002 @default.
• W2051208171 hasConcept C134018914 @default.
• W2051208171 hasConcept C160268369 @default.
• W2051208171 hasConcept C169760540 @default.
• W2051208171 hasConcept C170493617 @default.
• W2051208171 hasConcept C185592680 @default.
• W2051208171 hasConcept C189184402 @default.
• W2051208171 hasConcept C2776108384 @default.
• W2051208171 hasConcept C2779803574 @default.
• W2051208171 hasConcept C55493867 @default.
• W2051208171 hasConcept C61174792 @default.
• W2051208171 hasConcept C67018056 @default.
• W2051208171 hasConcept C71924100 @default.
• W2051208171 hasConcept C73009533 @default.
• W2051208171 hasConcept C86803240 @default.
• W2051208171 hasConceptScore W2051208171C12554922 @default.
• W2051208171 hasConceptScore W2051208171C126322002 @default.
• W2051208171 hasConceptScore W2051208171C134018914 @default.
• W2051208171 hasConceptScore W2051208171C160268369 @default.
• W2051208171 hasConceptScore W2051208171C169760540 @default.
• W2051208171 hasConceptScore W2051208171C170493617 @default.
• W2051208171 hasConceptScore W2051208171C185592680 @default.
• W2051208171 hasConceptScore W2051208171C189184402 @default.
• W2051208171 hasConceptScore W2051208171C2776108384 @default.
• W2051208171 hasConceptScore W2051208171C2779803574 @default.
• W2051208171 hasConceptScore W2051208171C55493867 @default.
• W2051208171 hasConceptScore W2051208171C61174792 @default.
• W2051208171 hasConceptScore W2051208171C67018056 @default.
• W2051208171 hasConceptScore W2051208171C71924100 @default.
• W2051208171 hasConceptScore W2051208171C73009533 @default.

• next