Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051213863> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2051213863 endingPage "1748" @default.
- W2051213863 startingPage "1745" @default.
- W2051213863 abstract "Treatment of DNA digests with nuclease P1 prior to 32P-labeling of adducts has previously been shown to enhance the sensitivity of the 32P-postlabeling assay for the detection of aromatic carcinogen-DNA adducts. The enhancement was based on the ability of nuclease P1 to remove the 3′-phosphate from normal nucleotides but not the corresponding phosphate from most aromatic adducted nucleotides. We investigated the utility of another 3′-dephosphorylating enzyme, nuclease S1, for this purpose, and found it to be as effective as nuclease P1. The recovery of DNA adducts derived from benzo[a]pyrene (B[a]P), benzoquinone (BQ) and 2-acetylaminofluorene (AAF) was comparable after enhancement with either enzyme. Some differences were, however, observed. Recovery of a minor B[a]P adduct was 1.5 times higher by the S1 procedure. Among minor adducts of BQ, two showed higher values (2.8- and 6.1-fold) by the S1 procedure and one by the P1 procedure (2.4-fold). The major AAF adduct, deoxyguanosine-C8-AF, exhibited poorer recovery (1–11%) by either procedure, while the minor adducts, deoxyguanosine-N2-AAF and deoxyguanosine-C8-AAF, showed better recovery (2–3 times) than by the enhancement procedure involving extraction of adducts into butanol. Our results show that the nuclease S1 assay can complement the nuclease P1 assay, with improved recoveries for some adducts. Considering the complexity of the postlabeling assay, this additional variant may prove useful in unequivocal detection of DNA adducts." @default.
- W2051213863 created "2016-06-24" @default.
- W2051213863 creator A5053562755 @default.
- W2051213863 date "1991-01-01" @default.
- W2051213863 modified "2023-10-14" @default.
- W2051213863 title "Nuclease S1-mediated enhancement of the 32P-postlabeling assay for aromatic carcinogen-DNA adducts" @default.
- W2051213863 doi "https://doi.org/10.1093/carcin/12.9.1745" @default.
- W2051213863 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1893535" @default.
- W2051213863 hasPublicationYear "1991" @default.
- W2051213863 type Work @default.
- W2051213863 sameAs 2051213863 @default.
- W2051213863 citedByCount "11" @default.
- W2051213863 crossrefType "journal-article" @default.
- W2051213863 hasAuthorship W2051213863A5053562755 @default.
- W2051213863 hasConcept C104317684 @default.
- W2051213863 hasConcept C108204754 @default.
- W2051213863 hasConcept C114246631 @default.
- W2051213863 hasConcept C139024627 @default.
- W2051213863 hasConcept C153911025 @default.
- W2051213863 hasConcept C178790620 @default.
- W2051213863 hasConcept C181199279 @default.
- W2051213863 hasConcept C185592680 @default.
- W2051213863 hasConcept C2777271071 @default.
- W2051213863 hasConcept C2779838395 @default.
- W2051213863 hasConcept C2780441211 @default.
- W2051213863 hasConcept C2781247443 @default.
- W2051213863 hasConcept C512185932 @default.
- W2051213863 hasConcept C552990157 @default.
- W2051213863 hasConcept C55493867 @default.
- W2051213863 hasConcept C83640560 @default.
- W2051213863 hasConcept C84772758 @default.
- W2051213863 hasConcept C86803240 @default.
- W2051213863 hasConcept C87644729 @default.
- W2051213863 hasConceptScore W2051213863C104317684 @default.
- W2051213863 hasConceptScore W2051213863C108204754 @default.
- W2051213863 hasConceptScore W2051213863C114246631 @default.
- W2051213863 hasConceptScore W2051213863C139024627 @default.
- W2051213863 hasConceptScore W2051213863C153911025 @default.
- W2051213863 hasConceptScore W2051213863C178790620 @default.
- W2051213863 hasConceptScore W2051213863C181199279 @default.
- W2051213863 hasConceptScore W2051213863C185592680 @default.
- W2051213863 hasConceptScore W2051213863C2777271071 @default.
- W2051213863 hasConceptScore W2051213863C2779838395 @default.
- W2051213863 hasConceptScore W2051213863C2780441211 @default.
- W2051213863 hasConceptScore W2051213863C2781247443 @default.
- W2051213863 hasConceptScore W2051213863C512185932 @default.
- W2051213863 hasConceptScore W2051213863C552990157 @default.
- W2051213863 hasConceptScore W2051213863C55493867 @default.
- W2051213863 hasConceptScore W2051213863C83640560 @default.
- W2051213863 hasConceptScore W2051213863C84772758 @default.
- W2051213863 hasConceptScore W2051213863C86803240 @default.
- W2051213863 hasConceptScore W2051213863C87644729 @default.
- W2051213863 hasIssue "9" @default.
- W2051213863 hasLocation W20512138631 @default.
- W2051213863 hasLocation W20512138632 @default.
- W2051213863 hasOpenAccess W2051213863 @default.
- W2051213863 hasPrimaryLocation W20512138631 @default.
- W2051213863 hasRelatedWork W1977145576 @default.
- W2051213863 hasRelatedWork W2002727295 @default.
- W2051213863 hasRelatedWork W2017299876 @default.
- W2051213863 hasRelatedWork W2023428439 @default.
- W2051213863 hasRelatedWork W2024808455 @default.
- W2051213863 hasRelatedWork W2051213863 @default.
- W2051213863 hasRelatedWork W2128442121 @default.
- W2051213863 hasRelatedWork W2139700768 @default.
- W2051213863 hasRelatedWork W2147984984 @default.
- W2051213863 hasRelatedWork W2149602703 @default.
- W2051213863 hasVolume "12" @default.
- W2051213863 isParatext "false" @default.
- W2051213863 isRetracted "false" @default.
- W2051213863 magId "2051213863" @default.
- W2051213863 workType "article" @default.