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• W2051215995 endingPage "599" @default.
• W2051215995 startingPage "571" @default.
• W2051215995 abstract "1. Some kinetic aspects of renal tubular acidification were studied in proximal and distal tubules of the rat kidney by combining stationary microperfusion methods and continuous measurements of luminal pH changes of phosphate or bicarbonate buffers by means of antimony electrodes. The analysis included the measurement of steady-state pH, steady-state buffer concentrations and acidification half-times. From these data, net rates of tubular bicarbonate reabsorption and of H ion secretion were obtained since it was shown that the rate of phosphate acidification provides a realistic estimate of H ion secretion. 2. Experiments were performed in control rats, in animals undergoing metabolic acidosis or alkalosis and in control and acidotic rats receiving the carbonic anydrase inhibitor Diamox. 3. In all experiments, the rates of tubular bicarbonate reabsorption and of phosphate acidification (H ion secretion) were proportional to luminal buffer levels. The changes of luminal acid concentrations followed first-order kinetics. 4. Steady-state transepithelial pH differences were reduced in metabolic alkalosis and after diamox but augmented during metabolic acidosis. 5. Acidification half-times were prolonged in metabolic acidosis and after Diamox but remained similar to control levels in metabolic alkalosis. 6. From the observation that both bicarbonate reabsorption and phosphate acidification are similarly affected by these experimental manoeuvres, it is concluded that H ion secretion plays a key role in both transport processes." @default.
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• W2051215995 date "1977-06-01" @default.
• W2051215995 modified "2023-10-15" @default.
• W2051215995 title "Kinetics of luminal acidification in cortical tubules of the rat kidney." @default.
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• W2051215995 doi "https://doi.org/10.1113/jphysiol.1977.sp011827" @default.
• W2051215995 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1283629" @default.
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