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- W2051221343 abstract "Abstract An antisense class I patatin gene under control of the CaMV 35S promoter was introduced into potato ( Solanum tuberosum ) cultivar E-potato 3 using the Agrobacterium tumefaciens system. PCR amplification and PCR–Southern blot analysis indicated that the antisense class I patatin gene had been integrated into the potato genome. Northern hybridization analysis showed that the antisense gene transcribed normally in the transgenic potato plants and resulted in a reduction of endogenous class I patatin mRNA. Total soluble protein content and lipid acyl hydrolase activity of microtubers, derived from transformed plants, decreased by a maximum of 36.4% and 31.4%, respectively, compared with control plants. The expression of this antisense gene also resulted in reductions of the plantlets forming tubers, tubers per plantlet and the effective tubers (≥50 mg) of the transformed plants." @default.
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- W2051221343 date "2005-04-01" @default.
- W2051221343 modified "2023-09-25" @default.
- W2051221343 title "Transformation of potato using an antisense class I patatin gene and its effect on microtuber formation" @default.
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- W2051221343 doi "https://doi.org/10.1079/cjb200544" @default.
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