FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051222820> ?p ?o ?g. }

• W2051222820 endingPage "106" @default.
• W2051222820 startingPage "100" @default.
• W2051222820 abstract "During the passage through the epididymis, testicular spermatozoa are directly exposed to epididymal fluid and undergo maturation. Proteins and glycoproteins of epididymal fluid may be adsorbed on the sperm surface and participate in the sperm maturation process, potentially in sperm capacitation, gamete recognition, binding and fusion. In present study, we separated proteins from boar epididymal fluid and tested their binding abilities. Boar epididymal fluid proteins were separated by size exclusion chromatography and by high-performance liquid chromatography with reverse phase (RP HPLC). The protein fractions were characterized by SDS-electrophoresis and the electrophoretic separated proteins after transfer to nitrocellulose membranes were tested for the interaction with biotin-labeled ligands: glycoproteins of zona pellucida (ZP), hyaluronic acid and heparin. Simultaneously, changes in the interaction of epididymal spermatozoa with biotin-labeled ligands after pre-incubation with epididymal fluid fractions were studied on microtiter plates by the ELBA (enzyme-linked binding assay) test. The affinity of some low-molecular-mass epididymal proteins (12–17 kDa and 23 kDa) to heparin and hyaluronic acid suggests their binding ability to oviductal proteoglycans of the porcine oviduct and a possible role during sperm capacitation. Epididymal proteins of 12–18 kDa interacted with ZP glycoproteins. One of them was identified as Crisp3-like protein. The method using microtiter plates showed the ability of epididymal fluid fractions to change the interaction of the epididymal sperm surface with biotin-labeled ligands (ZP glycoproteins, hyaluronic acid and heparin). These findings indicate that some epididymal fluid proteins are bound to the sperm surface during epididymal maturation and might play a role in the sperm capacitation or the sperm–zona pellucida binding." @default.
• W2051222820 created "2016-06-24" @default.
• W2051222820 creator A5049526881 @default.
• W2051222820 creator A5065001915 @default.
• W2051222820 creator A5069362592 @default.
• W2051222820 date "2011-01-01" @default.
• W2051222820 modified "2023-10-15" @default.
• W2051222820 title "Biochemical and binding characteristics of boar epididymal fluid proteins" @default.
• W2051222820 cites W178201007 @default.
• W2051222820 cites W1871924278 @default.
• W2051222820 cites W1983454444 @default.
• W2051222820 cites W1999231417 @default.
• W2051222820 cites W2009092555 @default.
• W2051222820 cites W2009845536 @default.
• W2051222820 cites W2011613202 @default.
• W2051222820 cites W2025854334 @default.
• W2051222820 cites W2030644454 @default.
• W2051222820 cites W2035725571 @default.
• W2051222820 cites W2038086488 @default.
• W2051222820 cites W2053729697 @default.
• W2051222820 cites W2080030507 @default.
• W2051222820 cites W2084842582 @default.
• W2051222820 cites W2090488167 @default.
• W2051222820 cites W2092463586 @default.
• W2051222820 cites W2100837269 @default.
• W2051222820 cites W2101108802 @default.
• W2051222820 cites W2117308234 @default.
• W2051222820 cites W2133098842 @default.
• W2051222820 cites W2135782435 @default.
• W2051222820 cites W2139412501 @default.
• W2051222820 cites W2144130844 @default.
• W2051222820 cites W2151095994 @default.
• W2051222820 cites W2163027453 @default.
• W2051222820 cites W2164578937 @default.
• W2051222820 cites W4236952805 @default.
• W2051222820 cites W9121732 @default.
• W2051222820 doi "https://doi.org/10.1016/j.jchromb.2010.11.023" @default.
• W2051222820 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21163710" @default.
• W2051222820 hasPublicationYear "2011" @default.
• W2051222820 type Work @default.
• W2051222820 sameAs 2051222820 @default.
• W2051222820 citedByCount "9" @default.
• W2051222820 countsByYear W20512228202013 @default.
• W2051222820 countsByYear W20512228202017 @default.
• W2051222820 countsByYear W20512228202018 @default.
• W2051222820 countsByYear W20512228202019 @default.
• W2051222820 countsByYear W20512228202021 @default.
• W2051222820 countsByYear W20512228202022 @default.
• W2051222820 countsByYear W20512228202023 @default.
• W2051222820 crossrefType "journal-article" @default.
• W2051222820 hasAuthorship W2051222820A5049526881 @default.
• W2051222820 hasAuthorship W2051222820A5065001915 @default.
• W2051222820 hasAuthorship W2051222820A5069362592 @default.
• W2051222820 hasConcept C108625454 @default.
• W2051222820 hasConcept C185592680 @default.
• W2051222820 hasConcept C196843134 @default.
• W2051222820 hasConcept C202751555 @default.
• W2051222820 hasConcept C2776010925 @default.
• W2051222820 hasConcept C2776690073 @default.
• W2051222820 hasConcept C2776803348 @default.
• W2051222820 hasConcept C2777359717 @default.
• W2051222820 hasConcept C2780301668 @default.
• W2051222820 hasConcept C2781087480 @default.
• W2051222820 hasConcept C2781254305 @default.
• W2051222820 hasConcept C43617362 @default.
• W2051222820 hasConcept C55493867 @default.
• W2051222820 hasConcept C59822182 @default.
• W2051222820 hasConcept C86803240 @default.
• W2051222820 hasConcept C95444343 @default.
• W2051222820 hasConceptScore W2051222820C108625454 @default.
• W2051222820 hasConceptScore W2051222820C185592680 @default.
• W2051222820 hasConceptScore W2051222820C196843134 @default.
• W2051222820 hasConceptScore W2051222820C202751555 @default.
• W2051222820 hasConceptScore W2051222820C2776010925 @default.
• W2051222820 hasConceptScore W2051222820C2776690073 @default.
• W2051222820 hasConceptScore W2051222820C2776803348 @default.
• W2051222820 hasConceptScore W2051222820C2777359717 @default.
• W2051222820 hasConceptScore W2051222820C2780301668 @default.
• W2051222820 hasConceptScore W2051222820C2781087480 @default.
• W2051222820 hasConceptScore W2051222820C2781254305 @default.
• W2051222820 hasConceptScore W2051222820C43617362 @default.
• W2051222820 hasConceptScore W2051222820C55493867 @default.
• W2051222820 hasConceptScore W2051222820C59822182 @default.
• W2051222820 hasConceptScore W2051222820C86803240 @default.
• W2051222820 hasConceptScore W2051222820C95444343 @default.
• W2051222820 hasFunder F4320321005 @default.
• W2051222820 hasFunder F4320321006 @default.
• W2051222820 hasFunder F4320322482 @default.
• W2051222820 hasIssue "1" @default.
• W2051222820 hasLocation W20512228201 @default.
• W2051222820 hasLocation W20512228202 @default.
• W2051222820 hasOpenAccess W2051222820 @default.
• W2051222820 hasPrimaryLocation W20512228201 @default.
• W2051222820 hasRelatedWork W1994267643 @default.
• W2051222820 hasRelatedWork W2004359705 @default.
• W2051222820 hasRelatedWork W2011827107 @default.
• W2051222820 hasRelatedWork W2027840448 @default.
• W2051222820 hasRelatedWork W2051887312 @default.

• next