FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051223485> ?p ?o ?g. }

• W2051223485 endingPage "847" @default.
• W2051223485 startingPage "840" @default.
• W2051223485 abstract "It has been demonstrated that administration of an interleukin-1 receptor antagonist protein (IL-1ra) reduces ischemic brain injury; however, the detrimental mechanism initiated by interleukin-1 (IL-1) in ischemic brain injury is unclear. In this study, we used mice that were transfected to overexpress human IL-1ra to elucidate the role of IL-1 in the activation of the inflammatory response after middle cerebral artery occlusion (MCAO). Myeloperoxidase (MPO) activity and immunohistostaining were used as a marker of polymorphonuclear leukocytes (PMNL) infiltration. Adenoviral vector (1 × 10 9 particles) was administered by injection into the right lateral ventricle in mice. Five days later, MCAO was performed on the mice using a suture technique. Permanent MCAO was achieved for 24 hours in the Ad.RSV IL-1ra-transfected, Ad.RSV lacZ-transfected, and saline (control) mice. Myeloperoxidase activity was quantified in each region and localization of MPO was determined by immunohistochemistry. After 2 hours of MCAO, the surface cerebral blood flow was reduced to 13.5% ± 3.4%, 10.75% ± 2.6%, and 10.9% ± 2.6% of baseline in the ischemic hemisphere in Ad.RSV IL-1ra-transfected, Ad.RSV- lacZ-transfected, and saline-treated mice, respectively. The MPO activity in the ischemic hemisphere in the Ad.RSV lacZ group was similar to that in the saline control group (cortex: 0.40 ± 0.22 versus 0.33 ± 0.11; basal ganglia: 0.46 ± 0.23 versus 0.49 ± 0.17; P > 0.05); however, it was significantly reduced in the Ad.RSV IL-1ra group (cortex: 0.18 ± 0.07; basal ganglia: 0.26 ± 0.15; P < 0.05). Myeloperoxidase immunohistochemistry showed that the massive accumulation of MPO-positive cells in the ischemic cortex, striatum, and corpus callosum regions was greatly attenuated in Ad.RSV IL-1ra-transfected mice. Our results indicate that Ad.RSV IL-1ra-transfected mice provide a useful tool to study the mechanism of action of IL-1. The MPO activity assay and immunostaining after 24 hours of focal ischemia were significantly reduced in IL-1ra gene-transfected mice, suggesting that IL-1 may play an important role in the activation of inflammatory cells during focal cerebral ischemia." @default.
• W2051223485 created "2016-06-24" @default.
• W2051223485 creator A5001764400 @default.
• W2051223485 creator A5002551693 @default.
• W2051223485 creator A5011209390 @default.
• W2051223485 creator A5022662736 @default.
• W2051223485 creator A5044994052 @default.
• W2051223485 creator A5045163747 @default.
• W2051223485 creator A5068262111 @default.
• W2051223485 date "1998-08-01" @default.
• W2051223485 modified "2023-10-11" @default.
• W2051223485 title "Attenuation of Ischemic Inflammatory Response in Mouse Brain Using an Adenoviral Vector to Induce Overexpression of Interleukin-1 Receptor Antagonist" @default.
• W2051223485 cites W1482686873 @default.
• W2051223485 cites W1574330564 @default.
• W2051223485 cites W1690233168 @default.
• W2051223485 cites W191994213 @default.
• W2051223485 cites W1977821697 @default.
• W2051223485 cites W1979090235 @default.
• W2051223485 cites W1980369396 @default.
• W2051223485 cites W1986004576 @default.
• W2051223485 cites W1989658278 @default.
• W2051223485 cites W1989904261 @default.
• W2051223485 cites W1997255414 @default.
• W2051223485 cites W1997510003 @default.
• W2051223485 cites W1998200062 @default.
• W2051223485 cites W2000196140 @default.
• W2051223485 cites W2005035542 @default.
• W2051223485 cites W2006791697 @default.
• W2051223485 cites W2009957587 @default.
• W2051223485 cites W2014484891 @default.
• W2051223485 cites W2020358486 @default.
• W2051223485 cites W2021998799 @default.
• W2051223485 cites W2023396428 @default.
• W2051223485 cites W2029969090 @default.
• W2051223485 cites W2038123205 @default.
• W2051223485 cites W2045735396 @default.
• W2051223485 cites W2047817815 @default.
• W2051223485 cites W2048879756 @default.
• W2051223485 cites W2056397762 @default.
• W2051223485 cites W2065527973 @default.
• W2051223485 cites W2067436359 @default.
• W2051223485 cites W2073099321 @default.
• W2051223485 cites W2074393911 @default.
• W2051223485 cites W2076398948 @default.
• W2051223485 cites W2079908353 @default.
• W2051223485 cites W2080562431 @default.
• W2051223485 cites W2080685344 @default.
• W2051223485 cites W2086129363 @default.
• W2051223485 cites W2091111800 @default.
• W2051223485 cites W2137232579 @default.
• W2051223485 cites W2148000616 @default.
• W2051223485 cites W2156953890 @default.
• W2051223485 cites W2167915117 @default.
• W2051223485 cites W2240012954 @default.
• W2051223485 doi "https://doi.org/10.1097/00004647-199808000-00004" @default.
• W2051223485 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9701345" @default.
• W2051223485 hasPublicationYear "1998" @default.
• W2051223485 type Work @default.
• W2051223485 sameAs 2051223485 @default.
• W2051223485 citedByCount "75" @default.
• W2051223485 countsByYear W20512234852012 @default.
• W2051223485 countsByYear W20512234852013 @default.
• W2051223485 countsByYear W20512234852014 @default.
• W2051223485 countsByYear W20512234852015 @default.
• W2051223485 countsByYear W20512234852016 @default.
• W2051223485 countsByYear W20512234852019 @default.
• W2051223485 countsByYear W20512234852021 @default.
• W2051223485 crossrefType "journal-article" @default.
• W2051223485 hasAuthorship W2051223485A5001764400 @default.
• W2051223485 hasAuthorship W2051223485A5002551693 @default.
• W2051223485 hasAuthorship W2051223485A5011209390 @default.
• W2051223485 hasAuthorship W2051223485A5022662736 @default.
• W2051223485 hasAuthorship W2051223485A5044994052 @default.
• W2051223485 hasAuthorship W2051223485A5045163747 @default.
• W2051223485 hasAuthorship W2051223485A5068262111 @default.
• W2051223485 hasBestOaLocation W20512234851 @default.
• W2051223485 hasConcept C126322002 @default.
• W2051223485 hasConcept C134018914 @default.
• W2051223485 hasConcept C142724271 @default.
• W2051223485 hasConcept C149892131 @default.
• W2051223485 hasConcept C170493617 @default.
• W2051223485 hasConcept C203014093 @default.
• W2051223485 hasConcept C203565725 @default.
• W2051223485 hasConcept C2776885963 @default.
• W2051223485 hasConcept C2776914184 @default.
• W2051223485 hasConcept C2778122271 @default.
• W2051223485 hasConcept C71924100 @default.
• W2051223485 hasConcept C98274493 @default.
• W2051223485 hasConceptScore W2051223485C126322002 @default.
• W2051223485 hasConceptScore W2051223485C134018914 @default.
• W2051223485 hasConceptScore W2051223485C142724271 @default.
• W2051223485 hasConceptScore W2051223485C149892131 @default.
• W2051223485 hasConceptScore W2051223485C170493617 @default.
• W2051223485 hasConceptScore W2051223485C203014093 @default.
• W2051223485 hasConceptScore W2051223485C203565725 @default.
• W2051223485 hasConceptScore W2051223485C2776885963 @default.
• W2051223485 hasConceptScore W2051223485C2776914184 @default.
• W2051223485 hasConceptScore W2051223485C2778122271 @default.

• next