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- W2051224734 abstract "To compare safety and efficiency in vitrification of mouse and human embryos between the use of closed type carrier (Rapid-i) and open type carrier (Cryoloop). A prospective study. Eight-cell and blastocyst stages mouse embryos were vitrified-warmed using Cryoloop and Rapid-I carriers, and then cultured to compare the safety of the carriers with the survival and developmental rates of the mouse embryos. TUNEL assay was performed to assess DNA damage of the mouse embryos vitrified-warmed. Of the 267 human embryos vitrified-warmed on day 3 using Cryoloop and Rapid-I, 179 embryos were transferred. The efficiency of the carriers was investigated by comparison of the survival and pregnancy rates of the human embryos. The survival rates of 8-cell and blastocyst stage embryos vitrified-warmed by using the Rapid-I (99.8% and 96.7%, respectively) were comparable to those obtained by using the Cryoloop (100% and 97.4%, respectively). Blastulation rates of the 8-cell stage embryos vitrified-warmed by Cryoloop and Rapid-I were 89.9 and 93.2%, respectively. Hatched out rates of the mouse blastocysts vitrified-warmed by Cryoloop and Rapid-I were 68.9 and 70.2%, respectively. Quantitative analysis for DNA damage of the mouse embryos showed that there was no difference in the percentage of TUNEL positive cells (DNA damaged cells) between the Cryoloop and Rapid-I groups. Although the intact survival rate of human embryos vitrified-warmed by Cryoloop (79.7%) was significantly higher than the rate of Rapid-I (64.3%, p<0.05), there was no difference in the clinical pregnancy rate between the Cryoloop (50%) and Rapid-I (54%) groups. We confirmed the safety of the Rapid-I carriers with the high survival and developmental rates of the vitrified-warmed mouse embryos. Moreover the efficiency of the Rapid-I in the vitrification of human embryos was confirmed by the stable clinical pregnancy rate. Therefore, we suggest that use of the closed type carrier could maintain the proper pregnancy rate without the potential risk of cross contamination." @default.
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- W2051224734 date "2014-09-01" @default.
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- W2051224734 title "Comparison of the safety and efficiency in vitrification of mouse and human embryos between open device-cryoloop and closed device-Rapid-I" @default.
- W2051224734 doi "https://doi.org/10.1016/j.fertnstert.2014.07.1099" @default.
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