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• W2051224854 abstract "The Bacillus sphaericus gene encoding penicillin V amidase, which catalyzes the hydrolysis of penicillin V, has been characterized. The entire nucleotide sequence of the coding region, as well as 5'- and 3'-flanking regions, was determined using an improved sequencing strategy. The deduced amino acid sequence suggests a protein consisting of 338 residues with an Mr of 37,500. The ATG initiator codon is preceded by a putative ribosome-binding site, typical for genes of Gram-positive origin. High expression of the gene was obtained in Escherichia coli using an inducible promoter, showing that the gene product is stable in this heterologous host." @default.
• W2051224854 created "2016-06-24" @default.
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• W2051224854 date "1986-01-01" @default.
• W2051224854 modified "2023-10-15" @default.
• W2051224854 title "Sequencing and heterologous expression of the gene encoding penicillin V amidase from Bacillus sphaericus" @default.
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• W2051224854 doi "https://doi.org/10.1016/0378-1119(86)90252-0" @default.
• W2051224854 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3026906" @default.
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