FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051226833> ?p ?o ?g. }

• W2051226833 endingPage "955" @default.
• W2051226833 startingPage "939" @default.
• W2051226833 abstract "Aerosol delivery of plasmid DNA therapeutic solutions is promising for the treatment of respiratory diseases. However, it poses challenges, most significantly the need to protect the delicate supercoiled (sc) structure of plasmid during aerosolization. Nebulizers for liquid aerosolization using meshes appear a better method for delivery than conventional jet and ultrasonic nebulizers. This paper explores their application to the delivery of plasmid DNA. A computational fluid dynamics model of the dynamics of fluid flow through the nozzle of the MicroAIR mesh nebulizer indicated high strain rates (>10(5) s(-1)) near the nozzle exit capable of causing damage to the shear-sensitive plasmid DNA. Knowledge of the strain rates predicted using CFD and molecule size determined using atomic force microscopy (AFM) enabled estimation of the hydrodynamic force and whether damage of shear-sensitive therapeutics was likely. Plasmids of size 5.7 and 20 kb were aerosolized in the mesh nebulizer. The sc structure of the 5.7-kb plasmid was successfully delivered without damage, while aerosolization of the 20-kb plasmid led to disintegration of the pDNA sc structure as observed in AFM. Subsequent formulation of the sc 20-kb plasmid with PEI resulted in successful aerosol delivery. The maximum hydrodynamic forces computed for the aerosolization of structures of the size of 5.7-kb and PEI formulated 20-kb plasmids were less than the forces reported to damage the structure of double-stranded DNA. A combination of CFD analysis and structure analysis may be used to predict successful aerosol delivery in such a mesh nebulizer." @default.
• W2051226833 created "2016-06-24" @default.
• W2051226833 creator A5002262055 @default.
• W2051226833 creator A5025572547 @default.
• W2051226833 creator A5054346972 @default.
• W2051226833 creator A5069677902 @default.
• W2051226833 creator A5069976221 @default.
• W2051226833 date "2007-01-01" @default.
• W2051226833 modified "2023-10-02" @default.
• W2051226833 title "Studies on aerosol delivery of plasmid DNA using a mesh nebulizer" @default.
• W2051226833 cites W1520821663 @default.
• W2051226833 cites W1545421925 @default.
• W2051226833 cites W1964441323 @default.
• W2051226833 cites W1967768293 @default.
• W2051226833 cites W1970419257 @default.
• W2051226833 cites W1973838623 @default.
• W2051226833 cites W1984471128 @default.
• W2051226833 cites W1985475454 @default.
• W2051226833 cites W1986749528 @default.
• W2051226833 cites W1993457635 @default.
• W2051226833 cites W1993882958 @default.
• W2051226833 cites W1994508480 @default.
• W2051226833 cites W2007146866 @default.
• W2051226833 cites W2008126601 @default.
• W2051226833 cites W2015242294 @default.
• W2051226833 cites W2024055277 @default.
• W2051226833 cites W2024316435 @default.
• W2051226833 cites W2026127150 @default.
• W2051226833 cites W2027032874 @default.
• W2051226833 cites W2035236521 @default.
• W2051226833 cites W2043736119 @default.
• W2051226833 cites W2044524458 @default.
• W2051226833 cites W2064968166 @default.
• W2051226833 cites W2067740988 @default.
• W2051226833 cites W2068500455 @default.
• W2051226833 cites W2080752439 @default.
• W2051226833 cites W2084476395 @default.
• W2051226833 cites W2096887947 @default.
• W2051226833 cites W2103503110 @default.
• W2051226833 cites W2110897008 @default.
• W2051226833 cites W2114161854 @default.
• W2051226833 cites W2115759544 @default.
• W2051226833 cites W2116934953 @default.
• W2051226833 cites W2133499175 @default.
• W2051226833 cites W2142095271 @default.
• W2051226833 cites W2148660248 @default.
• W2051226833 doi "https://doi.org/10.1002/bit.21493" @default.
• W2051226833 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17497741" @default.
• W2051226833 hasPublicationYear "2007" @default.
• W2051226833 type Work @default.
• W2051226833 sameAs 2051226833 @default.
• W2051226833 citedByCount "46" @default.
• W2051226833 countsByYear W20512268332012 @default.
• W2051226833 countsByYear W20512268332013 @default.
• W2051226833 countsByYear W20512268332014 @default.
• W2051226833 countsByYear W20512268332015 @default.
• W2051226833 countsByYear W20512268332016 @default.
• W2051226833 countsByYear W20512268332017 @default.
• W2051226833 countsByYear W20512268332018 @default.
• W2051226833 countsByYear W20512268332019 @default.
• W2051226833 countsByYear W20512268332020 @default.
• W2051226833 countsByYear W20512268332021 @default.
• W2051226833 countsByYear W20512268332022 @default.
• W2051226833 countsByYear W20512268332023 @default.
• W2051226833 crossrefType "journal-article" @default.
• W2051226833 hasAuthorship W2051226833A5002262055 @default.
• W2051226833 hasAuthorship W2051226833A5025572547 @default.
• W2051226833 hasAuthorship W2051226833A5054346972 @default.
• W2051226833 hasAuthorship W2051226833A5069677902 @default.
• W2051226833 hasAuthorship W2051226833A5069976221 @default.
• W2051226833 hasConcept C105702510 @default.
• W2051226833 hasConcept C121332964 @default.
• W2051226833 hasConcept C171250308 @default.
• W2051226833 hasConcept C185592680 @default.
• W2051226833 hasConcept C192562407 @default.
• W2051226833 hasConcept C22744801 @default.
• W2051226833 hasConcept C2776954882 @default.
• W2051226833 hasConcept C2780352487 @default.
• W2051226833 hasConcept C42219234 @default.
• W2051226833 hasConcept C552990157 @default.
• W2051226833 hasConcept C55493867 @default.
• W2051226833 hasConcept C56200935 @default.
• W2051226833 hasConcept C71924100 @default.
• W2051226833 hasConcept C82085835 @default.
• W2051226833 hasConcept C86803240 @default.
• W2051226833 hasConcept C97355855 @default.
• W2051226833 hasConceptScore W2051226833C105702510 @default.
• W2051226833 hasConceptScore W2051226833C121332964 @default.
• W2051226833 hasConceptScore W2051226833C171250308 @default.
• W2051226833 hasConceptScore W2051226833C185592680 @default.
• W2051226833 hasConceptScore W2051226833C192562407 @default.
• W2051226833 hasConceptScore W2051226833C22744801 @default.
• W2051226833 hasConceptScore W2051226833C2776954882 @default.
• W2051226833 hasConceptScore W2051226833C2780352487 @default.
• W2051226833 hasConceptScore W2051226833C42219234 @default.
• W2051226833 hasConceptScore W2051226833C552990157 @default.
• W2051226833 hasConceptScore W2051226833C55493867 @default.
• W2051226833 hasConceptScore W2051226833C56200935 @default.

• next