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• W2051228320 abstract "In this report, we demonstrate a microfluidic platform to control the stalk contraction and extension of Vorticella convallaria by changing concentration of Ca2+ with pneumatically-actuated elastomeric microvalves. Habitation, extraction and control of V. convallaria were carried out in a PDMS-based microfluidic device. By treating the cells with the permeant saponin, external actuation of cell-anchoring stalk between an extended and contracted state was achieved by cyclic exposure of the cells to a Ca2+ buffer (10(-6) M) and a rinse buffer containing EGTA as a chelation agent. When solutions were switched, the stalk contracted and extended responding to the ambient Ca2+ concentration change. The length of the stalk changed between 20 and 60 microm, resulting in a working distance of about 40 microm." @default.
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• W2051228320 date "2010-01-01" @default.
• W2051228320 modified "2023-10-06" @default.
• W2051228320 title "Chemical control of Vorticella bioactuator using microfluidics" @default.
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• W2051228320 doi "https://doi.org/10.1039/c003427d" @default.
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