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• W2051229799 endingPage "e32011" @default.
• W2051229799 startingPage "e32011" @default.
• W2051229799 abstract "Promiscuity, the basis for the evolution of new functions through ‘tinkering’ of residues in the vicinity of the catalytic site, is yet to be quantitatively defined. We present a computational method Promiscuity Indices Estimator (PROMISE) - based on signatures derived from the spatial and electrostatic properties of the catalytic residues, to estimate the promiscuity (PromIndex) of proteins with known active site residues and 3D structure. PromIndex reflects the number of different active site signatures that have congruent matches in close proximity of its native catalytic site, the quality of the matches and difference in the enzymatic activity. Promiscuity in proteins is observed to follow a lognormal distribution (μ = 0.28, σ = 1.1 reduced chi-square = 3.0E-5). The PROMISE predicted promiscuous functions in any protein can serve as the starting point for directed evolution experiments. PROMISE ranks carboxypeptidase A and ribonuclease A amongst the more promiscuous proteins. We have also investigated the properties of the residues in the vicinity of the catalytic site that regulates its promiscuity. Linear regression establishes a weak correlation (R2∼0.1) between certain properties of the residues (charge, polar, etc) in the neighborhood of the catalytic residues and PromIndex. A stronger relationship states that most proteins with high promiscuity have high percentages of charged and polar residues within a radius of 3 Å of the catalytic site, which is validated using one-tailed hypothesis tests (P-values∼0.05). Since it is known that these characteristics are key factors in catalysis, their relationship with the promiscuity index cross validates the methodology of PROMISE." @default.
• W2051229799 created "2016-06-24" @default.
• W2051229799 creator A5037521623 @default.
• W2051229799 creator A5066407334 @default.
• W2051229799 date "2012-02-16" @default.
• W2051229799 modified "2023-09-27" @default.
• W2051229799 title "A Measure of the Promiscuity of Proteins and Characteristics of Residues in the Vicinity of the Catalytic Site That Regulate Promiscuity" @default.
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• W2051229799 cites W1964640688 @default.
• W2051229799 cites W1967414045 @default.
• W2051229799 cites W1969589458 @default.
• W2051229799 cites W1970710213 @default.
• W2051229799 cites W1971099088 @default.
• W2051229799 cites W1972125501 @default.
• W2051229799 cites W1981574164 @default.
• W2051229799 cites W1982792504 @default.
• W2051229799 cites W1984351113 @default.
• W2051229799 cites W1986969007 @default.
• W2051229799 cites W1987696643 @default.
• W2051229799 cites W1989966591 @default.
• W2051229799 cites W1992625839 @default.
• W2051229799 cites W1996049679 @default.
• W2051229799 cites W1996142946 @default.
• W2051229799 cites W1996423252 @default.
• W2051229799 cites W1998014636 @default.
• W2051229799 cites W1998238795 @default.
• W2051229799 cites W1998966098 @default.
• W2051229799 cites W2003788979 @default.
• W2051229799 cites W2003890287 @default.
• W2051229799 cites W2007978853 @default.
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• W2051229799 doi "https://doi.org/10.1371/journal.pone.0032011" @default.
• W2051229799 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3281107" @default.
• W2051229799 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22359655" @default.
• W2051229799 hasPublicationYear "2012" @default.
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