FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051233989> ?p ?o ?g. }

• W2051233989 endingPage "tw14" @default.
• W2051233989 startingPage "tw14" @default.
• W2051233989 abstract "Nerve growth factor (NGF) binds to two structurally unrelated cell surface receptors, the p75 neurotrophin receptor (p75) and the receptor tyrosine kinase TrkA. p75 increases TrkA’s responsiveness to NGF, and coexpression of the two receptors appears to lead to the formation of binding sites with higher affinity for NGF than that of either receptor alone. This suggests that the high-affinity NGF-binding site may involve a complex containing both p75 and TrkA. Wehrmann et al . determined the crystal structure of the complete TrkA extracellular domain in complex with NGF and found that this structure was not incompatible with the existence of a 1:2:1 TrkA-NGF-p75 ternary complex. To investigate this possibility in a cellular context, the authors determined interactions between proteins (p75 and a truncated form of TrkA) fused to fragments of β-galactosidase (which become functional when brought together) that were expressed in C2C12 myoblasts. NGF promoted formation of TrkA homodimers but not that of TrkA-p75 heterodimers (which also did not exist in untreated cells); p75 formed oligomers in the absence of ligand, and NGF did not affect this oligomerization. When corrected for background binding, Scatchard analysis of 125 I-NGF binding in both PC12 cells and C2C12 cells expressing TrkA and p75 constructs failed to reveal the higher-affinity NGF binding site. Thus, the authors conclude that functional interactions between p75 and TrkA likely depend on downstream signaling rather than physical complexes involving the two receptors. In discussing this research, Barker suggests that p75 could pass NGF to TrkA in a ternary complex that exists too briefly to be detected by the β-galactosidase complementation assay. T. Wehrman, X. He, B. Raab, A. Dukipatti, H. Blau, K. C. Garcia, Structural and mechanistic insights into nerve growth factor interactions with the TrkA and p75 receptors. Neuron 53 , 25-38 (2007). [PubMed] P. A. Barker, High affinity not in the vicinity? Neuron 53 , 1-4 (2007). [PubMed]" @default.
• W2051233989 created "2016-06-24" @default.
• W2051233989 creator A5077591824 @default.
• W2051233989 date "2007-01-09" @default.
• W2051233989 modified "2023-09-23" @default.
• W2051233989 title "How Do TrkA and p75 Interact?" @default.
• W2051233989 doi "https://doi.org/10.1126/stke.3682007tw14" @default.
• W2051233989 hasPublicationYear "2007" @default.
• W2051233989 type Work @default.
• W2051233989 sameAs 2051233989 @default.
• W2051233989 citedByCount "0" @default.
• W2051233989 crossrefType "journal-article" @default.
• W2051233989 hasAuthorship W2051233989A5077591824 @default.
• W2051233989 hasConcept C144133560 @default.
• W2051233989 hasConcept C170493617 @default.
• W2051233989 hasConcept C171034665 @default.
• W2051233989 hasConcept C2778423431 @default.
• W2051233989 hasConcept C54355233 @default.
• W2051233989 hasConcept C56703729 @default.
• W2051233989 hasConcept C86803240 @default.
• W2051233989 hasConceptScore W2051233989C144133560 @default.
• W2051233989 hasConceptScore W2051233989C170493617 @default.
• W2051233989 hasConceptScore W2051233989C171034665 @default.
• W2051233989 hasConceptScore W2051233989C2778423431 @default.
• W2051233989 hasConceptScore W2051233989C54355233 @default.
• W2051233989 hasConceptScore W2051233989C56703729 @default.
• W2051233989 hasConceptScore W2051233989C86803240 @default.
• W2051233989 hasIssue "368" @default.
• W2051233989 hasLocation W20512339891 @default.
• W2051233989 hasOpenAccess W2051233989 @default.
• W2051233989 hasPrimaryLocation W20512339891 @default.
• W2051233989 hasRelatedWork W2018358004 @default.
• W2051233989 hasRelatedWork W2041698104 @default.
• W2051233989 hasRelatedWork W2046231665 @default.
• W2051233989 hasRelatedWork W2051233989 @default.
• W2051233989 hasRelatedWork W2069876079 @default.
• W2051233989 hasRelatedWork W2073167608 @default.
• W2051233989 hasRelatedWork W2357404056 @default.
• W2051233989 hasRelatedWork W2891283359 @default.
• W2051233989 hasRelatedWork W3179114317 @default.
• W2051233989 hasRelatedWork W748976835 @default.
• W2051233989 hasVolume "2007" @default.
• W2051233989 isParatext "false" @default.
• W2051233989 isRetracted "false" @default.
• W2051233989 magId "2051233989" @default.
• W2051233989 workType "article" @default.