FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051234617> ?p ?o ?g. }

• W2051234617 endingPage "222" @default.
• W2051234617 startingPage "215" @default.
• W2051234617 abstract "The Escherichia coli AmtB protein is member of the ubiquitous Amt family of ammonium transporters. Using a variety of [14C]methylammonium-uptake assays in wild-type E. coli, together with amtB and glutamine synthetase (glnA) mutants, we have shown that the filtration method traditionally used to measure [14C]methylammonium uptake actually measures intracellular accumulation of methylglutamine and that the kinetic data deduced from such experiments refer to the activity of glutamine synthetase and not to AmtB. Furthermore, the marked difference between the Km values of glutamine synthetase calculated in vitro and those calculated in vivo from our data suggest that ammonium assimilation by glutamine synthetase is coupled to the function of AmtB. The use of a modified assay technique allows us to measure AmtB activity in vivo. In this way, we have examined the role that AmtB plays in ammonium/methylammonium transport, in the light of conflicting proposals with regard to both the mode of action of Amt proteins and their substrate, i.e. ammonia or ammonium. Our in vivo data suggest that AmtB acts as a slowly conducting channel for NH3 that is neither dependent on the membrane potential nor on ATP. Furthermore, studies on competition between ammonium and methylammonium suggest that AmtB has a binding site for NH4+ on the periplasmic face." @default.
• W2051234617 created "2016-06-24" @default.
• W2051234617 creator A5041957934 @default.
• W2051234617 creator A5048527032 @default.
• W2051234617 creator A5058026562 @default.
• W2051234617 creator A5064864720 @default.
• W2051234617 creator A5071488584 @default.
• W2051234617 date "2005-08-09" @default.
• W2051234617 modified "2023-10-16" @default.
• W2051234617 title "<i>In vivo</i> functional characterization of the <i>Escherichia coli</i> ammonium channel AmtB: evidence for metabolic coupling of AmtB to glutamine synthetase" @default.
• W2051234617 cites W1488538240 @default.
• W2051234617 cites W1536381247 @default.
• W2051234617 cites W1537700204 @default.
• W2051234617 cites W1590327503 @default.
• W2051234617 cites W1600495850 @default.
• W2051234617 cites W1613664118 @default.
• W2051234617 cites W1761707887 @default.
• W2051234617 cites W1781531090 @default.
• W2051234617 cites W1828949911 @default.
• W2051234617 cites W1877828639 @default.
• W2051234617 cites W1914699704 @default.
• W2051234617 cites W1980596212 @default.
• W2051234617 cites W1983589176 @default.
• W2051234617 cites W1989302103 @default.
• W2051234617 cites W1994088425 @default.
• W2051234617 cites W1995087505 @default.
• W2051234617 cites W1995419987 @default.
• W2051234617 cites W1999421879 @default.
• W2051234617 cites W2000456081 @default.
• W2051234617 cites W2007698137 @default.
• W2051234617 cites W2021832701 @default.
• W2051234617 cites W2041500621 @default.
• W2051234617 cites W2042717435 @default.
• W2051234617 cites W2046174197 @default.
• W2051234617 cites W2062249432 @default.
• W2051234617 cites W2066459649 @default.
• W2051234617 cites W2074231563 @default.
• W2051234617 cites W2089762048 @default.
• W2051234617 cites W2091470498 @default.
• W2051234617 cites W2093547534 @default.
• W2051234617 cites W2093638357 @default.
• W2051234617 cites W2107534710 @default.
• W2051234617 cites W2118099055 @default.
• W2051234617 cites W2122436542 @default.
• W2051234617 cites W2127270784 @default.
• W2051234617 cites W2153588923 @default.
• W2051234617 cites W2155960002 @default.
• W2051234617 cites W2159594922 @default.
• W2051234617 cites W2160924308 @default.
• W2051234617 cites W2163788802 @default.
• W2051234617 cites W2172111004 @default.
• W2051234617 cites W2288983040 @default.
• W2051234617 cites W3207926443 @default.
• W2051234617 doi "https://doi.org/10.1042/bj20042094" @default.
• W2051234617 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1184577" @default.
• W2051234617 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15876187" @default.
• W2051234617 hasPublicationYear "2005" @default.
• W2051234617 type Work @default.
• W2051234617 sameAs 2051234617 @default.
• W2051234617 citedByCount "90" @default.
• W2051234617 countsByYear W20512346172013 @default.
• W2051234617 countsByYear W20512346172014 @default.
• W2051234617 countsByYear W20512346172015 @default.
• W2051234617 countsByYear W20512346172016 @default.
• W2051234617 countsByYear W20512346172017 @default.
• W2051234617 countsByYear W20512346172018 @default.
• W2051234617 countsByYear W20512346172019 @default.
• W2051234617 countsByYear W20512346172020 @default.
• W2051234617 countsByYear W20512346172021 @default.
• W2051234617 countsByYear W20512346172022 @default.
• W2051234617 countsByYear W20512346172023 @default.
• W2051234617 crossrefType "journal-article" @default.
• W2051234617 hasAuthorship W2051234617A5041957934 @default.
• W2051234617 hasAuthorship W2051234617A5048527032 @default.
• W2051234617 hasAuthorship W2051234617A5058026562 @default.
• W2051234617 hasAuthorship W2051234617A5064864720 @default.
• W2051234617 hasAuthorship W2051234617A5071488584 @default.
• W2051234617 hasBestOaLocation W20512346172 @default.
• W2051234617 hasConcept C104317684 @default.
• W2051234617 hasConcept C12554922 @default.
• W2051234617 hasConcept C178790620 @default.
• W2051234617 hasConcept C185592680 @default.
• W2051234617 hasConcept C190114821 @default.
• W2051234617 hasConcept C201663137 @default.
• W2051234617 hasConcept C2779289688 @default.
• W2051234617 hasConcept C2779349466 @default.
• W2051234617 hasConcept C2779870022 @default.
• W2051234617 hasConcept C515207424 @default.
• W2051234617 hasConcept C547475151 @default.
• W2051234617 hasConcept C55493867 @default.
• W2051234617 hasConcept C86803240 @default.
• W2051234617 hasConceptScore W2051234617C104317684 @default.
• W2051234617 hasConceptScore W2051234617C12554922 @default.
• W2051234617 hasConceptScore W2051234617C178790620 @default.
• W2051234617 hasConceptScore W2051234617C185592680 @default.
• W2051234617 hasConceptScore W2051234617C190114821 @default.
• W2051234617 hasConceptScore W2051234617C201663137 @default.
• W2051234617 hasConceptScore W2051234617C2779289688 @default.

• next