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• W2051243013 endingPage "075101" @default.
• W2051243013 startingPage "075101" @default.
• W2051243013 abstract "Elucidation of unknown target proteins of a drug is of great importance in understanding cell biology and drug discovery. There have been extensive studies to discover and identify target proteins in the cell. Visualization of targets using drug-conjugated probes has been an important approach to gathering mechanistic information of drug action at the cellular level. As quantum dot (QD) nanocrystals have attracted much attention as a fluorescent probe in the bioimaging area, we prepared drug-conjugated QD to explore the potential of target discovery. As a model drug, we selected a well-known anticancer drug, methotrexate (MTX), which has been known to target dihydrofolate reductase (DHFR) with high affinity binding (K(d) = 0.54 nM). MTX molecules were covalently attached to amino-PEG-polymer-coated QDs. Specific interactions of MTX-conjugated QDs with DHFR were identified using agarose gel electrophoresis and fluorescence microscopy. Cellular uptake of the MTX-conjugated QDs in living CHO cells was investigated with regard to their localization and distribution pattern. MTX-QD was found to be internalized into the cells via caveolae-medicated endocytosis without significant sequestration in endosomes. A colocalization experiment of the MTX-QD conjugate with antiDHFR-TAT-QD also confirmed that MTX-QD binds to the target DHFR. This study showed the potential of the drug-QD conjugate to identify or visualize drug-target interactions in the cell, which is currently of great importance in the area of drug discovery and chemical biology." @default.
• W2051243013 created "2016-06-24" @default.
• W2051243013 creator A5004550953 @default.
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• W2051243013 date "2013-01-28" @default.
• W2051243013 modified "2023-10-16" @default.
• W2051243013 title "Synthesis, characterization and target protein binding of drug-conjugated quantum dotsin vitroand in living cells" @default.
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• W2051243013 doi "https://doi.org/10.1088/0957-4484/24/7/075101" @default.
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• W2051243013 hasPublicationYear "2013" @default.
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