FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051247418> ?p ?o ?g. }

• W2051247418 endingPage "2601" @default.
• W2051247418 startingPage "2593" @default.
• W2051247418 abstract "A novel therapeutic approach for the treatment of bone defects is gene therapy assisted bone tissue engineering using bone marrow stromal cells (hBMSC). The aim of this study was to investigate the influence of human epidermal growth factor (hEGF) on proliferation and alkaline phosphatase (AP) activity of primary hBMSC in vitro. hBMSC cultures were achieved by explantation culture of bone chips. Following exposure to 0-10 ng recombinant hEGF (rhEGF)/ml cell numbers were determined by automated cell counting and cell bound AP activity was measured spectrophotometrically. hBMSC were transfected with hEGF plasmids and the proliferative effect was studied by cocultivation of transfected and untreated cells using porous cell culture inserts. The persistence of hEGF expression even after cell transfer was studied by the generation of possibly osteogenic constructs introducing transfected hBMSC in fibrin glue and bovine cancellous bone. The maximum increase in proliferation (156 +/- 7%) and AP activity (220 +/- 34%) was detected after exposition to 10 ng rhEGF/ml. In the separation chamber assay transfected cells produced hEGF concentrations up to 3.6 ng/ml, which induced a mean proliferation increase of 93% which could be significantly inhibited by a neutralizing hEGF antibody. Further, EGFsecretion of transfected hBMSC in 3D-culture was verified. Recombinant and transgenic hEGF stimulate proliferation of primary hBMSC in vitro. Lipotransfection of hBMSC with hEGF plasmids allows the transient and site directed delivery of biologically active transgenic hEGF. The introduction of mitogenic, angiogenic and chemoattractive factors in gene therapy assisted bone tissue engineering is discussed by the example of EGF." @default.
• W2051247418 created "2016-06-24" @default.
• W2051247418 creator A5007649177 @default.
• W2051247418 creator A5008569109 @default.
• W2051247418 creator A5027186086 @default.
• W2051247418 creator A5034133648 @default.
• W2051247418 creator A5039904139 @default.
• W2051247418 creator A5041392918 @default.
• W2051247418 creator A5068621433 @default.
• W2051247418 date "2008-12-03" @default.
• W2051247418 modified "2023-10-12" @default.
• W2051247418 title "Epidermal growth factor (EGF) transfection of human bone marrow stromal cells in bone tissue engineering" @default.
• W2051247418 cites W1522229466 @default.
• W2051247418 cites W1880405183 @default.
• W2051247418 cites W1970661184 @default.
• W2051247418 cites W1974207011 @default.
• W2051247418 cites W1974223809 @default.
• W2051247418 cites W1975578801 @default.
• W2051247418 cites W1977355870 @default.
• W2051247418 cites W1982316160 @default.
• W2051247418 cites W1989485116 @default.
• W2051247418 cites W1989917319 @default.
• W2051247418 cites W1993871115 @default.
• W2051247418 cites W1994665876 @default.
• W2051247418 cites W2001297140 @default.
• W2051247418 cites W2005050639 @default.
• W2051247418 cites W2005734571 @default.
• W2051247418 cites W2006811526 @default.
• W2051247418 cites W2008021821 @default.
• W2051247418 cites W2014043168 @default.
• W2051247418 cites W2025918052 @default.
• W2051247418 cites W2044193051 @default.
• W2051247418 cites W2045705524 @default.
• W2051247418 cites W2047292103 @default.
• W2051247418 cites W2065418754 @default.
• W2051247418 cites W2078126231 @default.
• W2051247418 cites W2079281282 @default.
• W2051247418 cites W2083549141 @default.
• W2051247418 cites W2086943348 @default.
• W2051247418 cites W2092113319 @default.
• W2051247418 cites W2097097666 @default.
• W2051247418 cites W2132584956 @default.
• W2051247418 cites W2144268541 @default.
• W2051247418 cites W2147721391 @default.
• W2051247418 cites W2168902269 @default.
• W2051247418 cites W2169341720 @default.
• W2051247418 cites W4211147424 @default.
• W2051247418 cites W4295836001 @default.
• W2051247418 doi "https://doi.org/10.1111/j.1582-4934.2008.00600.x" @default.
• W2051247418 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6529967" @default.
• W2051247418 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19067768" @default.
• W2051247418 hasPublicationYear "2008" @default.
• W2051247418 type Work @default.
• W2051247418 sameAs 2051247418 @default.
• W2051247418 citedByCount "14" @default.
• W2051247418 countsByYear W20512474182013 @default.
• W2051247418 countsByYear W20512474182014 @default.
• W2051247418 countsByYear W20512474182015 @default.
• W2051247418 countsByYear W20512474182019 @default.
• W2051247418 countsByYear W20512474182020 @default.
• W2051247418 countsByYear W20512474182023 @default.
• W2051247418 crossrefType "journal-article" @default.
• W2051247418 hasAuthorship W2051247418A5007649177 @default.
• W2051247418 hasAuthorship W2051247418A5008569109 @default.
• W2051247418 hasAuthorship W2051247418A5027186086 @default.
• W2051247418 hasAuthorship W2051247418A5034133648 @default.
• W2051247418 hasAuthorship W2051247418A5039904139 @default.
• W2051247418 hasAuthorship W2051247418A5041392918 @default.
• W2051247418 hasAuthorship W2051247418A5068621433 @default.
• W2051247418 hasBestOaLocation W20512474181 @default.
• W2051247418 hasConcept C153911025 @default.
• W2051247418 hasConcept C16685009 @default.
• W2051247418 hasConcept C16930146 @default.
• W2051247418 hasConcept C185592680 @default.
• W2051247418 hasConcept C203014093 @default.
• W2051247418 hasConcept C2776362946 @default.
• W2051247418 hasConcept C2780007613 @default.
• W2051247418 hasConcept C28328180 @default.
• W2051247418 hasConcept C502942594 @default.
• W2051247418 hasConcept C54009773 @default.
• W2051247418 hasConcept C54355233 @default.
• W2051247418 hasConcept C55493867 @default.
• W2051247418 hasConcept C62112901 @default.
• W2051247418 hasConcept C71924100 @default.
• W2051247418 hasConcept C81885089 @default.
• W2051247418 hasConcept C86803240 @default.
• W2051247418 hasConcept C95444343 @default.
• W2051247418 hasConceptScore W2051247418C153911025 @default.
• W2051247418 hasConceptScore W2051247418C16685009 @default.
• W2051247418 hasConceptScore W2051247418C16930146 @default.
• W2051247418 hasConceptScore W2051247418C185592680 @default.
• W2051247418 hasConceptScore W2051247418C203014093 @default.
• W2051247418 hasConceptScore W2051247418C2776362946 @default.
• W2051247418 hasConceptScore W2051247418C2780007613 @default.
• W2051247418 hasConceptScore W2051247418C28328180 @default.
• W2051247418 hasConceptScore W2051247418C502942594 @default.
• W2051247418 hasConceptScore W2051247418C54009773 @default.
• W2051247418 hasConceptScore W2051247418C54355233 @default.

• next