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- W2051249046 abstract "Abstract 1. A study has been made of step-wise and gradient development in anion exchange chromatography. 2. In one-step development the anomalous step previously studied has been shown to arise from the anions adsorbed on the column during equilibration. 3. The deformation of concentration gradients by passage through the column has been demonstrated. 4. By the use of a combined pH and concentration gradient with mixed buffers highly purifid bovine carbonic anhydrase has been split up into two active peaks, which are probably identical. 5. The possibilities of obtaining artificial protein peaks in one-step and gradient development chromatography on anion exchangers are discussed." @default.
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- W2051249046 date "1962-01-01" @default.
- W2051249046 modified "2023-09-23" @default.
- W2051249046 title "Artificial zoning in anion exchange chromatography" @default.
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- W2051249046 doi "https://doi.org/10.1016/s0021-9673(01)99295-7" @default.
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