FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051257857> ?p ?o ?g. }

• W2051257857 endingPage "112" @default.
• W2051257857 startingPage "101" @default.
• W2051257857 abstract "We describe here an application of the competitive PCR technique to the analysis of copy number of recombinant rat parathyroid hormone-related protein (rPTHrP) gene in stably-transfected murine erythroleukemia (MEL) cell lines. A single-copy reference gene (endogenous mouse PTHrP gene or mPTHrP) is used as an internal control. This control gene, present in the genome of MEL cells, shares the same primer binding sites as the rPTHrP cDNA but contains an internal PvuII site, which allows resolution of the amplified products after restriction enzyme digestion by polyacrylamide gel electrophoresis (PAGE). The transgene copy number is determined by the ratio of band intensity of the rPTHrP product to that of the mPTHrP product. Using this method, we have determined the copy number of the rPTHrP transgene from isolated genomic DNA, and compared the results with those obtained from Southern blot analysis. In addition, we have demonstrated that the procedure can be applied very simply to whole MEL cells without DNA extractions and that as few as 10(4) cells are required for the analysis." @default.
• W2051257857 created "2016-06-24" @default.
• W2051257857 creator A5017567471 @default.
• W2051257857 creator A5024912541 @default.
• W2051257857 creator A5028606013 @default.
• W2051257857 creator A5034629575 @default.
• W2051257857 creator A5075679909 @default.
• W2051257857 date "1999-08-01" @default.
• W2051257857 modified "2023-09-22" @default.
• W2051257857 title "Rapid determination of transgene copy number in stably-transfected mammalian cells by competitive PCR" @default.
• W2051257857 cites W173196067 @default.
• W2051257857 cites W1963933110 @default.
• W2051257857 cites W1969267177 @default.
• W2051257857 cites W1975689068 @default.
• W2051257857 cites W1992964095 @default.
• W2051257857 cites W2043517126 @default.
• W2051257857 cites W2050206304 @default.
• W2051257857 cites W2093009120 @default.
• W2051257857 cites W2105041057 @default.
• W2051257857 cites W2106238604 @default.
• W2051257857 cites W2131780050 @default.
• W2051257857 cites W2138566067 @default.
• W2051257857 cites W2144634347 @default.
• W2051257857 cites W2146326776 @default.
• W2051257857 cites W2149897099 @default.
• W2051257857 cites W2156070837 @default.
• W2051257857 cites W2420389825 @default.
• W2051257857 cites W2493590780 @default.
• W2051257857 cites W2989345970 @default.
• W2051257857 doi "https://doi.org/10.1016/s0165-022x(99)00018-4" @default.
• W2051257857 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10487164" @default.
• W2051257857 hasPublicationYear "1999" @default.
• W2051257857 type Work @default.
• W2051257857 sameAs 2051257857 @default.
• W2051257857 citedByCount "2" @default.
• W2051257857 crossrefType "journal-article" @default.
• W2051257857 hasAuthorship W2051257857A5017567471 @default.
• W2051257857 hasAuthorship W2051257857A5024912541 @default.
• W2051257857 hasAuthorship W2051257857A5028606013 @default.
• W2051257857 hasAuthorship W2051257857A5034629575 @default.
• W2051257857 hasAuthorship W2051257857A5075679909 @default.
• W2051257857 hasConcept C102230213 @default.
• W2051257857 hasConcept C104317684 @default.
• W2051257857 hasConcept C116084860 @default.
• W2051257857 hasConcept C128319531 @default.
• W2051257857 hasConcept C133307689 @default.
• W2051257857 hasConcept C150194340 @default.
• W2051257857 hasConcept C153911025 @default.
• W2051257857 hasConcept C17757408 @default.
• W2051257857 hasConcept C178790620 @default.
• W2051257857 hasConcept C185592680 @default.
• W2051257857 hasConcept C187882448 @default.
• W2051257857 hasConcept C22744801 @default.
• W2051257857 hasConcept C2777116013 @default.
• W2051257857 hasConcept C2777563447 @default.
• W2051257857 hasConcept C40767141 @default.
• W2051257857 hasConcept C54009773 @default.
• W2051257857 hasConcept C54355233 @default.
• W2051257857 hasConcept C552990157 @default.
• W2051257857 hasConcept C55548728 @default.
• W2051257857 hasConcept C86803240 @default.
• W2051257857 hasConceptScore W2051257857C102230213 @default.
• W2051257857 hasConceptScore W2051257857C104317684 @default.
• W2051257857 hasConceptScore W2051257857C116084860 @default.
• W2051257857 hasConceptScore W2051257857C128319531 @default.
• W2051257857 hasConceptScore W2051257857C133307689 @default.
• W2051257857 hasConceptScore W2051257857C150194340 @default.
• W2051257857 hasConceptScore W2051257857C153911025 @default.
• W2051257857 hasConceptScore W2051257857C17757408 @default.
• W2051257857 hasConceptScore W2051257857C178790620 @default.
• W2051257857 hasConceptScore W2051257857C185592680 @default.
• W2051257857 hasConceptScore W2051257857C187882448 @default.
• W2051257857 hasConceptScore W2051257857C22744801 @default.
• W2051257857 hasConceptScore W2051257857C2777116013 @default.
• W2051257857 hasConceptScore W2051257857C2777563447 @default.
• W2051257857 hasConceptScore W2051257857C40767141 @default.
• W2051257857 hasConceptScore W2051257857C54009773 @default.
• W2051257857 hasConceptScore W2051257857C54355233 @default.
• W2051257857 hasConceptScore W2051257857C552990157 @default.
• W2051257857 hasConceptScore W2051257857C55548728 @default.
• W2051257857 hasConceptScore W2051257857C86803240 @default.
• W2051257857 hasIssue "3" @default.
• W2051257857 hasLocation W20512578571 @default.
• W2051257857 hasLocation W20512578572 @default.
• W2051257857 hasOpenAccess W2051257857 @default.
• W2051257857 hasPrimaryLocation W20512578571 @default.
• W2051257857 hasRelatedWork W2051257857 @default.
• W2051257857 hasRelatedWork W2055004041 @default.
• W2051257857 hasRelatedWork W2056172754 @default.
• W2051257857 hasRelatedWork W2080606595 @default.
• W2051257857 hasRelatedWork W2158738420 @default.
• W2051257857 hasRelatedWork W2338137066 @default.
• W2051257857 hasRelatedWork W2366367904 @default.
• W2051257857 hasRelatedWork W2375596579 @default.
• W2051257857 hasRelatedWork W2390231994 @default.
• W2051257857 hasRelatedWork W2608852418 @default.
• W2051257857 hasVolume "40" @default.
• W2051257857 isParatext "false" @default.

• next