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- W2051258336 abstract "We developed a novel copolymer modified amperometric immunosensor for the detection of cholera antitoxin (anti-CT), by the electropolymerization of pyrrole-biotin and pyrrole-lactitobionamide monomers on platinum or glassy carbon electrodes. In the detection of cholera antitoxin we have used three enzymatic marker detection systems based on HRP-labeled rabbit IgG antibodies, biotinylated polyphenol-oxidase (PPO-B) and biotinylated glucose-oxidase (GOX-B). The comparison of the electro-enzymatic performances of these three configurations with different substrates, clearly shows that the more sensitive amperometric immunosensor was based on HRP with a lower limit of detection of 50ng/ml anti-CT using hydroquinone/H(2)O(2) system. The response time for this substrate was in range of 5-30s. The HRP-amperometric immunosensor has thus proven to be a very sensitive tool to monitor nanomolar concentrations of anti-CT." @default.
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- W2051258336 date "2005-03-31" @default.
- W2051258336 modified "2023-10-07" @default.
- W2051258336 title "Comparison between the performances of amperometric immunosensors for cholera antitoxin based on three enzyme markers☆" @default.
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- W2051258336 doi "https://doi.org/10.1016/j.talanta.2004.06.048" @default.
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