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• W2051258449 abstract "Various attempts have been made to develop three-dimensional (3-D) cell culture methods because 3-D cells mimic the structures and functional properties of real tissue compared with those of monolayer cultures. Here, we report on a highly simple and efficient 3-D spheroid generation method based on a magnetic pin-array system to concentrate magnetic nanoparticle-incorporated cells in a focal direction. This system was comprised only of external magnets and magnetically induced iron pins to generate a concentrated magnetic field for attracting cells in a focused direction. 3-D spheroid generation was achieved simply by adding magnetic nanoparticle-incorporated cells into a well and covering the plate with a magnetic lid. Cell clustering occurred rapidly within 5 min and created more compact cells with time through the focused magnetic force. This system ensured not only reproducible and size-controlled generation of spheroids but also versatile types of spheroids such as random mixed, core-shell, and fused spheroids, providing a very useful tool for various biological applications." @default.
• W2051258449 created "2016-06-24" @default.
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• W2051258449 date "2013-11-01" @default.
• W2051258449 modified "2023-10-18" @default.
• W2051258449 title "High-throughput generation of spheroids using magnetic nanoparticles for three-dimensional cell culture" @default.
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• W2051258449 doi "https://doi.org/10.1016/j.biomaterials.2013.07.056" @default.
• W2051258449 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23937911" @default.
• W2051258449 hasPublicationYear "2013" @default.
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