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W2051264425 abstract "We present two FLIM endoscopes for clinical imaging and in vivo cell biology. For subcellular confocal imaging we demonstrated the first confocal FLIM endomicroscope, implementing TCSPC with a Cellvizio®GI, which we have now developed as a self-contained wheeled instrument (1.0 × 0.7 m) incorporating a tunable excitation laser and acquiring images in < 10 s. This has been applied to image FRET in live cells and to image tissue autofluorescence, for which we are implementing FIFO for image montaging. For diagnostic screening/guided biopsy, we have developed a complementary wide-field FLIM endoscope employing time-gated detection with violet and UV excitation for imaging over mm-cm fields of view." @default.
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W2051264425 date "2011-02-10" @default.
W2051264425 modified "2023-09-24" @default.
W2051264425 title "Fluorescence lifetime imaging endoscopy" @default.
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W2051264425 doi "https://doi.org/10.1117/12.874968" @default.
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