Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051269642> ?p ?o ?g. }
- W2051269642 endingPage "1085" @default.
- W2051269642 startingPage "1075" @default.
- W2051269642 abstract "We studied autoinhibition produced immediately after synaptic serotonin (5-HT) release in identified leech Retzius neurons, cultured singly or forming synapses onto pressure-sensitive neurons. Cultured Retzius neurons are isopotential, thus allowing accurate recordings of synaptic events using intracellular microelectrodes. The effects of autoinhibition on distant neuropilar presynaptic endings were predicted from model simulations. Following action potentials (APs), cultured neurons produced a slow hyperpolarization with a rise time of 85.4 ± 5.2 ms and a half-decay time of 252 ± 17.4 ms. These inhibitory postpotentials were reproduced by the iontophoretic application of 5-HT and became depolarizing after inverting the transmembranal chloride gradient by using microelectrodes filled with potassium chloride. The inhibitory postpotentials were reversibly abolished in the absence of extracellular calcium and absent in reserpine-treated neurons, suggesting an autoinhibition due to 5-HT acting on autoreceptors coupled to chloride channels. The autoinhibitory responses increased the membrane conductance and decreased subsequent excitability. Increasing 5-HT release by stimulating with trains of ten pulses at 10 or 30 Hz produced 23 ± 6 and 47 ± 2% of AP failures, respectively. These failures were reversibly abolished by the serotonergic antagonist methysergide (140 μM). Moreover, reserpine-treated neurons had only 5 ± 4% of failures during trains at 10 Hz. This percentage was increased to 35 ± 4% by iontophoretic application of 5-HT. Increases in AP failures correlated with smaller postsynaptic currents. Model simulations predicted that the autoinhibitory chloride conductance reduces the amplitude of APs arriving at neuropilar presynaptic endings. Altogether, our results suggest that 5-HT autoinhibits its subsequent release by decreasing the excitability of presynaptic endings within the same neuron." @default.
- W2051269642 created "2016-06-24" @default.
- W2051269642 creator A5071489794 @default.
- W2051269642 creator A5079461312 @default.
- W2051269642 creator A5081888798 @default.
- W2051269642 date "2009-08-01" @default.
- W2051269642 modified "2023-10-01" @default.
- W2051269642 title "Real-Time Measurements of Synaptic Autoinhibition Produced by Serotonin Release in Cultured Leech Neurons" @default.
- W2051269642 cites W1662108418 @default.
- W2051269642 cites W1665113494 @default.
- W2051269642 cites W1749481733 @default.
- W2051269642 cites W1762877386 @default.
- W2051269642 cites W1784856208 @default.
- W2051269642 cites W1824548881 @default.
- W2051269642 cites W1872585649 @default.
- W2051269642 cites W1896855389 @default.
- W2051269642 cites W1902428143 @default.
- W2051269642 cites W1964042817 @default.
- W2051269642 cites W1964423555 @default.
- W2051269642 cites W1965409765 @default.
- W2051269642 cites W1966579748 @default.
- W2051269642 cites W1967992123 @default.
- W2051269642 cites W1989049022 @default.
- W2051269642 cites W1989904990 @default.
- W2051269642 cites W1991461979 @default.
- W2051269642 cites W1991649616 @default.
- W2051269642 cites W1994922559 @default.
- W2051269642 cites W1995528444 @default.
- W2051269642 cites W1997312703 @default.
- W2051269642 cites W2000398693 @default.
- W2051269642 cites W2001003931 @default.
- W2051269642 cites W2005115756 @default.
- W2051269642 cites W2006189627 @default.
- W2051269642 cites W2011633019 @default.
- W2051269642 cites W2014996020 @default.
- W2051269642 cites W2020253685 @default.
- W2051269642 cites W2022460284 @default.
- W2051269642 cites W2026417579 @default.
- W2051269642 cites W2030431779 @default.
- W2051269642 cites W2033347211 @default.
- W2051269642 cites W2034961437 @default.
- W2051269642 cites W2039397636 @default.
- W2051269642 cites W2040799187 @default.
- W2051269642 cites W2041220381 @default.
- W2051269642 cites W2041920752 @default.
- W2051269642 cites W2046084951 @default.
- W2051269642 cites W2049089794 @default.
- W2051269642 cites W2049590508 @default.
- W2051269642 cites W2052853977 @default.
- W2051269642 cites W2058885383 @default.
- W2051269642 cites W2060671316 @default.
- W2051269642 cites W2061222238 @default.
- W2051269642 cites W2064943540 @default.
- W2051269642 cites W2065245499 @default.
- W2051269642 cites W2065624008 @default.
- W2051269642 cites W2069461255 @default.
- W2051269642 cites W2073725845 @default.
- W2051269642 cites W2075183645 @default.
- W2051269642 cites W2075240088 @default.
- W2051269642 cites W2081047826 @default.
- W2051269642 cites W2085325217 @default.
- W2051269642 cites W2085947730 @default.
- W2051269642 cites W2086729362 @default.
- W2051269642 cites W2087739225 @default.
- W2051269642 cites W2091703221 @default.
- W2051269642 cites W2091889367 @default.
- W2051269642 cites W2095196015 @default.
- W2051269642 cites W2111698175 @default.
- W2051269642 cites W2114458666 @default.
- W2051269642 cites W2116609875 @default.
- W2051269642 cites W2123462704 @default.
- W2051269642 cites W2137675854 @default.
- W2051269642 cites W2144372508 @default.
- W2051269642 cites W2149700585 @default.
- W2051269642 cites W2150530114 @default.
- W2051269642 cites W2163101452 @default.
- W2051269642 cites W2166915621 @default.
- W2051269642 cites W2170741639 @default.
- W2051269642 cites W2171676899 @default.
- W2051269642 cites W2182040937 @default.
- W2051269642 cites W2270283363 @default.
- W2051269642 cites W2295625753 @default.
- W2051269642 cites W2335948309 @default.
- W2051269642 cites W2409870233 @default.
- W2051269642 cites W2416423182 @default.
- W2051269642 doi "https://doi.org/10.1152/jn.00107.2009" @default.
- W2051269642 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19535486" @default.
- W2051269642 hasPublicationYear "2009" @default.
- W2051269642 type Work @default.
- W2051269642 sameAs 2051269642 @default.
- W2051269642 citedByCount "10" @default.
- W2051269642 countsByYear W20512696422012 @default.
- W2051269642 countsByYear W20512696422014 @default.
- W2051269642 countsByYear W20512696422015 @default.
- W2051269642 countsByYear W20512696422021 @default.
- W2051269642 crossrefType "journal-article" @default.
- W2051269642 hasAuthorship W2051269642A5071489794 @default.
- W2051269642 hasAuthorship W2051269642A5079461312 @default.