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- W2051278659 abstract "Ocular surface reconstruction with autologous oral mucosal epithelium has attracted attention as a novel treatment strategy that avoids allograft rejection.To evaluate the absorption of ultraviolet (UV) A or B irradiation by human oral mucosal epithelium cultured on human amniotic membrane.Human oral mucosal and limbal epithelial cells were co-cultured on amniotic membrane with inactivated 3T3 fibroblasts. The cell sheets were also subjected to UV-A (365 nm) or UV-B (302 nm) irradiation at energy levels ranging from 50 to 800 microW/cm2, and the UV absorption rate was measured with a UV irradiation meter.Cultured oral mucosal epithelium had a structure with 3-5 layers of cells, consistent with the histological features of cultured corneal limbal epithelium after 4 weeks. The decrease in UV-A absorption of cultivated oral mucosal epithelium ranged from 25 to 36% of that for cultured corneal epithelium. The increase in UV-B absorption by cultured oral mucosal epithelium between 200 and 800 microW/cm2 was approximately 145% of that for cultured corneal limbal epithelium.Our data demonstrated that cultured oral mucosal epithelium has low UV-A and high UV-B absorption capacity as compared with those of cultured corneal epithelium, suggesting that oral mucosal epithelium can compensate for UV absorption of corneal epithelium." @default.
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- W2051278659 date "2006-01-01" @default.
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- W2051278659 title "UV Absorption in Human Oral Mucosal Epithelial Sheets for Ocular Surface Reconstruction" @default.
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- W2051278659 doi "https://doi.org/10.1159/000096230" @default.
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