Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051279831> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2051279831 endingPage "356" @default.
- W2051279831 startingPage "354" @default.
- W2051279831 abstract "This study investigated the evolution of trans-9 trans-11 conjugated linoleic acid (CLA) from cis-9 trans-11 CLA during methylation and its avoidance through a rapid base methylation of milk fat. The study examined three conditions shown to result in loss of cis-9 trans-11 CLA during methylation namely: temperature, methylation time, water contamination in old reagents and acidic conditions. Three techniques currently used for the conversion of milk fat into fatty acid methyl esters for analysis of CLA content by gas liquid chromatography and a fourth procedure designed to eliminate acidic conditions and to limit methylation temperature and time were used. The four methods were: (i) acidic methylation (AM); (ii) acidic and basic bimethylation with fresh reagents (FBM); (iii) acidic and basic bimethylation with pre-prepared reagents (PBM) and (iv) basic methylation (BM). Each regime was carried out on six milk samples over two periods and methylated 1 ml freeze-dried milk ( n =12 per regime). Total CLA was not different across methylation regimes (0·30 mg/ml). Isomer cis-9 trans-11 was higher ( P <0·01) with BM than the other regimes and lowest with AM: 21·2, 17·8, 18·8 and 14·7 mg/100 ml for BM, FBM, PBM and AM, respectively. The inverse relationship was shown for trans-9 trans-11 with higher ( P <0·001) amounts with AM than the other regimes and lowest with BM: 0·57, 2·55, 2·36 and 3·69 mg/100 ml for BM, FBM, PBM and AM, respectively. The trans-10 cis-12 isomer was also shown to alter with methylation procedure being higher ( P <0·001) with AM than the other regimes: 0·43, 0·47, 0·29 and 1·20 mg/100 ml for BM, FBM, PBM and AM, respectively. Validation with known CLA free fatty acid and triacylglycerol standards confirmed that AM resulted in conversion of cis-9 trans-11 to trans-9 trans-11, and also elevated trans-10 cis-12 whilst BM of triacylglycerol CLA did not isomerise cis-9 trans-11 and was comparable to FBM." @default.
- W2051279831 created "2016-06-24" @default.
- W2051279831 creator A5036913988 @default.
- W2051279831 creator A5073928250 @default.
- W2051279831 date "2008-08-01" @default.
- W2051279831 modified "2023-09-25" @default.
- W2051279831 title "Isomerisation of cis-9 trans-11 conjugated linoleic acid (CLA) to trans-9 trans-11 CLA during acidic methylation can be avoided by a rapid base catalysed methylation of milk fat" @default.
- W2051279831 cites W1978062798 @default.
- W2051279831 cites W2013397568 @default.
- W2051279831 cites W2088888450 @default.
- W2051279831 cites W2136313405 @default.
- W2051279831 doi "https://doi.org/10.1017/s0022029908003518" @default.
- W2051279831 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18680620" @default.
- W2051279831 hasPublicationYear "2008" @default.
- W2051279831 type Work @default.
- W2051279831 sameAs 2051279831 @default.
- W2051279831 citedByCount "23" @default.
- W2051279831 countsByYear W20512798312012 @default.
- W2051279831 countsByYear W20512798312016 @default.
- W2051279831 countsByYear W20512798312017 @default.
- W2051279831 countsByYear W20512798312018 @default.
- W2051279831 countsByYear W20512798312019 @default.
- W2051279831 countsByYear W20512798312020 @default.
- W2051279831 countsByYear W20512798312022 @default.
- W2051279831 countsByYear W20512798312023 @default.
- W2051279831 crossrefType "journal-article" @default.
- W2051279831 hasAuthorship W2051279831A5036913988 @default.
- W2051279831 hasAuthorship W2051279831A5073928250 @default.
- W2051279831 hasBestOaLocation W20512798312 @default.
- W2051279831 hasConcept C126661725 @default.
- W2051279831 hasConcept C134306372 @default.
- W2051279831 hasConcept C161790260 @default.
- W2051279831 hasConcept C178790620 @default.
- W2051279831 hasConcept C185592680 @default.
- W2051279831 hasConcept C2779780233 @default.
- W2051279831 hasConcept C2780809830 @default.
- W2051279831 hasConcept C33288867 @default.
- W2051279831 hasConcept C33923547 @default.
- W2051279831 hasConcept C40875361 @default.
- W2051279831 hasConcept C42058472 @default.
- W2051279831 hasConcept C43617362 @default.
- W2051279831 hasConcept C543025807 @default.
- W2051279831 hasConcept C552990157 @default.
- W2051279831 hasConcept C55493867 @default.
- W2051279831 hasConceptScore W2051279831C126661725 @default.
- W2051279831 hasConceptScore W2051279831C134306372 @default.
- W2051279831 hasConceptScore W2051279831C161790260 @default.
- W2051279831 hasConceptScore W2051279831C178790620 @default.
- W2051279831 hasConceptScore W2051279831C185592680 @default.
- W2051279831 hasConceptScore W2051279831C2779780233 @default.
- W2051279831 hasConceptScore W2051279831C2780809830 @default.
- W2051279831 hasConceptScore W2051279831C33288867 @default.
- W2051279831 hasConceptScore W2051279831C33923547 @default.
- W2051279831 hasConceptScore W2051279831C40875361 @default.
- W2051279831 hasConceptScore W2051279831C42058472 @default.
- W2051279831 hasConceptScore W2051279831C43617362 @default.
- W2051279831 hasConceptScore W2051279831C543025807 @default.
- W2051279831 hasConceptScore W2051279831C552990157 @default.
- W2051279831 hasConceptScore W2051279831C55493867 @default.
- W2051279831 hasIssue "3" @default.
- W2051279831 hasLocation W20512798311 @default.
- W2051279831 hasLocation W20512798312 @default.
- W2051279831 hasLocation W20512798313 @default.
- W2051279831 hasLocation W20512798314 @default.
- W2051279831 hasOpenAccess W2051279831 @default.
- W2051279831 hasPrimaryLocation W20512798311 @default.
- W2051279831 hasRelatedWork W1982945380 @default.
- W2051279831 hasRelatedWork W2028178401 @default.
- W2051279831 hasRelatedWork W2043439362 @default.
- W2051279831 hasRelatedWork W2089198347 @default.
- W2051279831 hasRelatedWork W2089602716 @default.
- W2051279831 hasRelatedWork W2094557306 @default.
- W2051279831 hasRelatedWork W2624342189 @default.
- W2051279831 hasRelatedWork W2953213056 @default.
- W2051279831 hasRelatedWork W4238592385 @default.
- W2051279831 hasRelatedWork W2079864336 @default.
- W2051279831 hasVolume "75" @default.
- W2051279831 isParatext "false" @default.
- W2051279831 isRetracted "false" @default.
- W2051279831 magId "2051279831" @default.
- W2051279831 workType "article" @default.