Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051283485> ?p ?o ?g. }
- W2051283485 endingPage "6053" @default.
- W2051283485 startingPage "6049" @default.
- W2051283485 abstract "After ovulation mammalian oocytes arrest in second meiotic metaphase. We asked whether the microtubules that comprise the meiotic spindle of mouse oocytes were stable or were undergoing rapid cycles of assembly and disassembly. Porcine brain tubulin, derivatized with biotin or x-rhodamine [5- (and -6)-carboxy-x-rhodamine], was microinjected into living oocytes. Biotinylated tubulin incorporated into the meiotic spindle to apparent equilibrium within 15 min. To assess quantitatively the rates of disassembly and assembly of the microtubules, small domains within the spindles of oocytes injected with x-rhodamine-tubulin were photobleached and their recovery was analyzed by digital imaging microscopy. Fluorescence recovery in the spindles was rapid and extensive, plateauing to an average of 83% at 4 min. The calculated half-time for turnover of the spindle microtubules was 77 sec. In contrast, fluorescence recovery of the spindle midbodies in telophase oocytes was much more limited, averaging approximately 22% at 4 min. These data indicate that most microtubules within the arrested metaphase spindle of the mouse oocyte undergo rapid cycles of assembly and disassembly. Microtubules of the telophase midbody are more stable." @default.
- W2051283485 created "2016-06-24" @default.
- W2051283485 creator A5002316771 @default.
- W2051283485 creator A5054956917 @default.
- W2051283485 creator A5060338506 @default.
- W2051283485 creator A5078260531 @default.
- W2051283485 date "1990-08-01" @default.
- W2051283485 modified "2023-09-27" @default.
- W2051283485 title "Microtubules in the metaphase-arrested mouse oocyte turn over rapidly." @default.
- W2051283485 cites W1539361761 @default.
- W2051283485 cites W1965404941 @default.
- W2051283485 cites W1980613624 @default.
- W2051283485 cites W1993265798 @default.
- W2051283485 cites W1995356251 @default.
- W2051283485 cites W1995746467 @default.
- W2051283485 cites W2019721152 @default.
- W2051283485 cites W2023584657 @default.
- W2051283485 cites W2027054401 @default.
- W2051283485 cites W2029321925 @default.
- W2051283485 cites W2034140820 @default.
- W2051283485 cites W2036664585 @default.
- W2051283485 cites W2058401708 @default.
- W2051283485 cites W2059623287 @default.
- W2051283485 cites W2060731014 @default.
- W2051283485 cites W2067173413 @default.
- W2051283485 cites W2068843702 @default.
- W2051283485 cites W2073057352 @default.
- W2051283485 cites W2084136914 @default.
- W2051283485 cites W2094168229 @default.
- W2051283485 cites W2109396399 @default.
- W2051283485 cites W2125240364 @default.
- W2051283485 cites W2130386608 @default.
- W2051283485 cites W2132472784 @default.
- W2051283485 cites W2150507107 @default.
- W2051283485 cites W2153128242 @default.
- W2051283485 cites W2158540512 @default.
- W2051283485 cites W2163704200 @default.
- W2051283485 cites W2167603737 @default.
- W2051283485 cites W2171194083 @default.
- W2051283485 cites W2406589013 @default.
- W2051283485 cites W2416776913 @default.
- W2051283485 cites W2750045786 @default.
- W2051283485 doi "https://doi.org/10.1073/pnas.87.16.6049" @default.
- W2051283485 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/54469" @default.
- W2051283485 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2385583" @default.
- W2051283485 hasPublicationYear "1990" @default.
- W2051283485 type Work @default.
- W2051283485 sameAs 2051283485 @default.
- W2051283485 citedByCount "51" @default.
- W2051283485 countsByYear W20512834852012 @default.
- W2051283485 countsByYear W20512834852013 @default.
- W2051283485 countsByYear W20512834852014 @default.
- W2051283485 countsByYear W20512834852018 @default.
- W2051283485 countsByYear W20512834852019 @default.
- W2051283485 countsByYear W20512834852020 @default.
- W2051283485 countsByYear W20512834852021 @default.
- W2051283485 countsByYear W20512834852022 @default.
- W2051283485 countsByYear W20512834852023 @default.
- W2051283485 crossrefType "journal-article" @default.
- W2051283485 hasAuthorship W2051283485A5002316771 @default.
- W2051283485 hasAuthorship W2051283485A5054956917 @default.
- W2051283485 hasAuthorship W2051283485A5060338506 @default.
- W2051283485 hasAuthorship W2051283485A5078260531 @default.
- W2051283485 hasBestOaLocation W20512834852 @default.
- W2051283485 hasConcept C104317684 @default.
- W2051283485 hasConcept C12554922 @default.
- W2051283485 hasConcept C141561535 @default.
- W2051283485 hasConcept C148196450 @default.
- W2051283485 hasConcept C1491633281 @default.
- W2051283485 hasConcept C164265269 @default.
- W2051283485 hasConcept C185592680 @default.
- W2051283485 hasConcept C196843134 @default.
- W2051283485 hasConcept C20418707 @default.
- W2051283485 hasConcept C2776690073 @default.
- W2051283485 hasConcept C30481170 @default.
- W2051283485 hasConcept C45472230 @default.
- W2051283485 hasConcept C47432576 @default.
- W2051283485 hasConcept C54355233 @default.
- W2051283485 hasConcept C70278784 @default.
- W2051283485 hasConcept C84425145 @default.
- W2051283485 hasConcept C85813293 @default.
- W2051283485 hasConcept C86803240 @default.
- W2051283485 hasConcept C93304396 @default.
- W2051283485 hasConcept C95444343 @default.
- W2051283485 hasConcept C9565192 @default.
- W2051283485 hasConceptScore W2051283485C104317684 @default.
- W2051283485 hasConceptScore W2051283485C12554922 @default.
- W2051283485 hasConceptScore W2051283485C141561535 @default.
- W2051283485 hasConceptScore W2051283485C148196450 @default.
- W2051283485 hasConceptScore W2051283485C1491633281 @default.
- W2051283485 hasConceptScore W2051283485C164265269 @default.
- W2051283485 hasConceptScore W2051283485C185592680 @default.
- W2051283485 hasConceptScore W2051283485C196843134 @default.
- W2051283485 hasConceptScore W2051283485C20418707 @default.
- W2051283485 hasConceptScore W2051283485C2776690073 @default.
- W2051283485 hasConceptScore W2051283485C30481170 @default.
- W2051283485 hasConceptScore W2051283485C45472230 @default.
- W2051283485 hasConceptScore W2051283485C47432576 @default.