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- W2051286404 abstract "A combination of atomic force microscopy (AFM) and near field scanning optical microscopy has been used to study domain formation in dipalmitoylphosphatidylcholine (DPPC)/cholesterol monolayers with cholesterol concentrations ranging from 0 to 50%. The results show a clear evolution from a mixture of liquid expanded and liquid condensed phases for cholesterol concentrations < 10% to a mixture of liquid expanded and two cholesterol-containing phases at intermediate concentrations, and finally to a single homogeneous liquid ordered phase for 33% cholesterol. Mixtures of the various phases are clearly identified by height differences in AFM and in some cases by fluorescence imaging for samples containing 0.5% BODIPY dye, which localizes preferentially in the more fluid phase. Note that fluorescence imaging, at least with the dye used here, is unable to distinguish between the cholesterol-rich and cholesterol-poor phases detected at intermediate cholesterol concentrations. The combination of fluorescence and AFM imaging provides a more complete picture of the phase evolution for cholesterol/DPPC monolayers than could be obtained by either technique alone, and presents substantial advantages over conventional fluorescence microscopy in that submicrometre-sized domains can be readily detected." @default.
- W2051286404 created "2016-06-24" @default.
- W2051286404 creator A5010450510 @default.
- W2051286404 creator A5067050358 @default.
- W2051286404 date "2002-02-01" @default.
- W2051286404 modified "2023-09-24" @default.
- W2051286404 title "Phase evolution in cholesterol/DPPC monolayers: atomic force microscopy and near field scanning optical microscopy studies" @default.
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- W2051286404 doi "https://doi.org/10.1046/j.0022-2720.2001.00982.x" @default.
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