FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051287493> ?p ?o ?g. }

• W2051287493 endingPage "250" @default.
• W2051287493 startingPage "236" @default.
• W2051287493 abstract "A membrane proteinase from Pseudomonas aeruginosa , called insulin-cleaving membrane proteinase (ICMP), was located in the outer membrane leaflet of the cell envelope. The enzyme is expressed early in the logarithmic phase parallel to the bacterial growth during growth on peptide rich media. It is located with its active center facing to the outermost side of the cell, because its whole activity could be measured in intact cells. The very labile membrane proteinase was solubilized by non-ionic detergents (Nonidet P-40, Triton X-100) and purified in its amphiphilic form to apparent homogeneity in SDS-PAGE by copper chelate chromatography and two subsequent chromatographic steps on Red-Sepharose CL-4B (yield 58.3%, purification factor 776.3). It consisted of a single polypeptide chain with a molecular mass of 44.6 kDa, determined by mass spectrometry. ICMP was characterized to be a metalloprotease with pH-optimum in the neutral range. The ICMP readily hydrolyzed Glu 13 -Ala 14 and Tyr 16 -Leu 17 bonds in the insulin B-chain. Phe 25 -Tyr 26 and His 10 -Leu 11 were secondary cleavage sites suggesting a primary specificity of the enzyme for hydrophobic or aromatic residues at P′ 1 -position. The ICMP differed from elastase, alkaline protease and LasA in its cleavage specificity, inhibition behavior and was immunologically diverse from elastase. The amino acid sequence of internal peptides showed no homologies with the known proteinases. This outer membrane proteinase was capable of specific cleavage of α and β fibrinogen chains. Among the p -nitroanilide substrates tested, substrates of plasminogen activator, complement convertase and kallikrein with arginine residues in the P 1 -subsite were the substrates best accepted, but they were only cleaved at a very low rate." @default.
• W2051287493 created "2016-06-24" @default.
• W2051287493 creator A5028698748 @default.
• W2051287493 creator A5046264547 @default.
• W2051287493 creator A5053045498 @default.
• W2051287493 creator A5066735399 @default.
• W2051287493 creator A5074204141 @default.
• W2051287493 creator A5086916036 @default.
• W2051287493 date "1999-08-01" @default.
• W2051287493 modified "2023-09-27" @default.
• W2051287493 title "Characterization and purification of an outer membrane metalloproteinase from Pseudomonas aeruginosa with fibrinogenolytic activity" @default.
• W2051287493 cites W1488354887 @default.
• W2051287493 cites W1494351834 @default.
• W2051287493 cites W1508751842 @default.
• W2051287493 cites W1530747269 @default.
• W2051287493 cites W1533964351 @default.
• W2051287493 cites W1539642192 @default.
• W2051287493 cites W1552333098 @default.
• W2051287493 cites W1571314096 @default.
• W2051287493 cites W1599125820 @default.
• W2051287493 cites W1606469152 @default.
• W2051287493 cites W1607046039 @default.
• W2051287493 cites W172315901 @default.
• W2051287493 cites W1849176629 @default.
• W2051287493 cites W1907990126 @default.
• W2051287493 cites W1949772429 @default.
• W2051287493 cites W1958474746 @default.
• W2051287493 cites W1964777564 @default.
• W2051287493 cites W1965028224 @default.
• W2051287493 cites W1965164207 @default.
• W2051287493 cites W1977878533 @default.
• W2051287493 cites W1980712809 @default.
• W2051287493 cites W1982456459 @default.
• W2051287493 cites W1985634686 @default.
• W2051287493 cites W1987409290 @default.
• W2051287493 cites W1992843823 @default.
• W2051287493 cites W2014477644 @default.
• W2051287493 cites W2014528878 @default.
• W2051287493 cites W2021275615 @default.
• W2051287493 cites W2027980091 @default.
• W2051287493 cites W2039801586 @default.
• W2051287493 cites W2056449834 @default.
• W2051287493 cites W2060651894 @default.
• W2051287493 cites W2061081100 @default.
• W2051287493 cites W206657617 @default.
• W2051287493 cites W2071171055 @default.
• W2051287493 cites W2076838043 @default.
• W2051287493 cites W2079749322 @default.
• W2051287493 cites W2084998268 @default.
• W2051287493 cites W2085436045 @default.
• W2051287493 cites W2089068964 @default.
• W2051287493 cites W2091286404 @default.
• W2051287493 cites W2101108802 @default.
• W2051287493 cites W2103947911 @default.
• W2051287493 cites W2143427122 @default.
• W2051287493 cites W2149383490 @default.
• W2051287493 cites W2160107586 @default.
• W2051287493 cites W2295126578 @default.
• W2051287493 cites W2315658840 @default.
• W2051287493 cites W81265627 @default.
• W2051287493 cites W955725593 @default.
• W2051287493 doi "https://doi.org/10.1016/s0925-4439(99)00040-x" @default.
• W2051287493 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10452958" @default.
• W2051287493 hasPublicationYear "1999" @default.
• W2051287493 type Work @default.
• W2051287493 sameAs 2051287493 @default.
• W2051287493 citedByCount "36" @default.
• W2051287493 countsByYear W20512874932012 @default.
• W2051287493 countsByYear W20512874932013 @default.
• W2051287493 countsByYear W20512874932015 @default.
• W2051287493 countsByYear W20512874932016 @default.
• W2051287493 countsByYear W20512874932017 @default.
• W2051287493 countsByYear W20512874932018 @default.
• W2051287493 countsByYear W20512874932021 @default.
• W2051287493 countsByYear W20512874932022 @default.
• W2051287493 countsByYear W20512874932023 @default.
• W2051287493 crossrefType "journal-article" @default.
• W2051287493 hasAuthorship W2051287493A5028698748 @default.
• W2051287493 hasAuthorship W2051287493A5046264547 @default.
• W2051287493 hasAuthorship W2051287493A5053045498 @default.
• W2051287493 hasAuthorship W2051287493A5066735399 @default.
• W2051287493 hasAuthorship W2051287493A5074204141 @default.
• W2051287493 hasAuthorship W2051287493A5086916036 @default.
• W2051287493 hasConcept C104317684 @default.
• W2051287493 hasConcept C146587185 @default.
• W2051287493 hasConcept C181199279 @default.
• W2051287493 hasConcept C185592680 @default.
• W2051287493 hasConcept C2775974274 @default.
• W2051287493 hasConcept C2776714187 @default.
• W2051287493 hasConcept C2778471508 @default.
• W2051287493 hasConcept C2780340462 @default.
• W2051287493 hasConcept C2781044401 @default.
• W2051287493 hasConcept C2781307694 @default.
• W2051287493 hasConcept C43617362 @default.
• W2051287493 hasConcept C547475151 @default.
• W2051287493 hasConcept C55493867 @default.
• W2051287493 hasConcept C55728118 @default.
• W2051287493 hasConceptScore W2051287493C104317684 @default.

• next