FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051296345> ?p ?o ?g. }

• W2051296345 endingPage "1160" @default.
• W2051296345 startingPage "1153" @default.
• W2051296345 abstract "Riboswitch aptamers adopt diverse and complex tertiary structural folds that contain both single-stranded and double-stranded regions. We observe that this high degree of secondary structure leads to an appreciable hypochromicity that is not accounted for in the standard method to calculate extinction coefficients using nearest-neighbor effects, which results in a systematic underestimation of RNA concentrations. Here we present a practical method for quantifying riboswitch RNAs using thermal hydrolysis to generate the corresponding pool of mononucleotides, for which precise extinction coefficients have been measured. Thermal hydrolysis can be performed at neutral pH without reaction quenching, avoids the use of nucleases or expensive fluorescent dyes, and does not require generation of calibration curves. The accuracy of this method for determining RNA concentrations has been validated using quantitative (31)P-NMR calibrated to an external standard. We expect that this simple procedure will be generally useful for the accurate quantification of any sequence-defined RNA sample, which is often a critical parameter for in vitro binding and kinetic assays." @default.
• W2051296345 created "2016-06-24" @default.
• W2051296345 creator A5000724016 @default.
• W2051296345 creator A5006081212 @default.
• W2051296345 creator A5033537158 @default.
• W2051296345 creator A5053309607 @default.
• W2051296345 date "2014-05-23" @default.
• W2051296345 modified "2023-09-25" @default.
• W2051296345 title "A neutral pH thermal hydrolysis method for quantification of structured RNAs" @default.
• W2051296345 cites W1027635430 @default.
• W2051296345 cites W1531875369 @default.
• W2051296345 cites W1964161187 @default.
• W2051296345 cites W1964261732 @default.
• W2051296345 cites W1995533382 @default.
• W2051296345 cites W1996252125 @default.
• W2051296345 cites W2002830509 @default.
• W2051296345 cites W2008753573 @default.
• W2051296345 cites W2008773545 @default.
• W2051296345 cites W2009370969 @default.
• W2051296345 cites W2012328151 @default.
• W2051296345 cites W2015293171 @default.
• W2051296345 cites W2020403806 @default.
• W2051296345 cites W2021429071 @default.
• W2051296345 cites W2021774273 @default.
• W2051296345 cites W2030443929 @default.
• W2051296345 cites W2030621944 @default.
• W2051296345 cites W2030817376 @default.
• W2051296345 cites W2034537520 @default.
• W2051296345 cites W2045401515 @default.
• W2051296345 cites W2048706341 @default.
• W2051296345 cites W2063331211 @default.
• W2051296345 cites W2066695319 @default.
• W2051296345 cites W2067364045 @default.
• W2051296345 cites W2084261354 @default.
• W2051296345 cites W2084695608 @default.
• W2051296345 cites W2086632920 @default.
• W2051296345 cites W2086977264 @default.
• W2051296345 cites W2088267546 @default.
• W2051296345 cites W2089388639 @default.
• W2051296345 cites W2335424249 @default.
• W2051296345 cites W2403912335 @default.
• W2051296345 cites W2413955605 @default.
• W2051296345 cites W3022614077 @default.
• W2051296345 doi "https://doi.org/10.1261/rna.045856.114" @default.
• W2051296345 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4114692" @default.
• W2051296345 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24860014" @default.
• W2051296345 hasPublicationYear "2014" @default.
• W2051296345 type Work @default.
• W2051296345 sameAs 2051296345 @default.
• W2051296345 citedByCount "25" @default.
• W2051296345 countsByYear W20512963452014 @default.
• W2051296345 countsByYear W20512963452015 @default.
• W2051296345 countsByYear W20512963452016 @default.
• W2051296345 countsByYear W20512963452017 @default.
• W2051296345 countsByYear W20512963452018 @default.
• W2051296345 countsByYear W20512963452019 @default.
• W2051296345 countsByYear W20512963452020 @default.
• W2051296345 countsByYear W20512963452021 @default.
• W2051296345 countsByYear W20512963452022 @default.
• W2051296345 countsByYear W20512963452023 @default.
• W2051296345 crossrefType "journal-article" @default.
• W2051296345 hasAuthorship W2051296345A5000724016 @default.
• W2051296345 hasAuthorship W2051296345A5006081212 @default.
• W2051296345 hasAuthorship W2051296345A5033537158 @default.
• W2051296345 hasAuthorship W2051296345A5053309607 @default.
• W2051296345 hasBestOaLocation W20512963451 @default.
• W2051296345 hasConcept C104317684 @default.
• W2051296345 hasConcept C128972844 @default.
• W2051296345 hasConcept C148625055 @default.
• W2051296345 hasConcept C186060115 @default.
• W2051296345 hasConcept C194993378 @default.
• W2051296345 hasConcept C54355233 @default.
• W2051296345 hasConcept C55493867 @default.
• W2051296345 hasConcept C67705224 @default.
• W2051296345 hasConcept C86803240 @default.
• W2051296345 hasConcept C94412978 @default.
• W2051296345 hasConceptScore W2051296345C104317684 @default.
• W2051296345 hasConceptScore W2051296345C128972844 @default.
• W2051296345 hasConceptScore W2051296345C148625055 @default.
• W2051296345 hasConceptScore W2051296345C186060115 @default.
• W2051296345 hasConceptScore W2051296345C194993378 @default.
• W2051296345 hasConceptScore W2051296345C54355233 @default.
• W2051296345 hasConceptScore W2051296345C55493867 @default.
• W2051296345 hasConceptScore W2051296345C67705224 @default.
• W2051296345 hasConceptScore W2051296345C86803240 @default.
• W2051296345 hasConceptScore W2051296345C94412978 @default.
• W2051296345 hasIssue "7" @default.
• W2051296345 hasLocation W20512963451 @default.
• W2051296345 hasLocation W20512963452 @default.
• W2051296345 hasLocation W20512963453 @default.
• W2051296345 hasLocation W20512963454 @default.
• W2051296345 hasOpenAccess W2051296345 @default.
• W2051296345 hasPrimaryLocation W20512963451 @default.
• W2051296345 hasRelatedWork W1021950895 @default.
• W2051296345 hasRelatedWork W1581465026 @default.
• W2051296345 hasRelatedWork W2176054603 @default.
• W2051296345 hasRelatedWork W2531153359 @default.
• W2051296345 hasRelatedWork W2680270385 @default.

• next