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- W2051342615 abstract "A new technique for separation of human colostral macrophages and neutrophils was developed. Macrophages attached more readily to acid soluble collagen-serum or gelatin-serum substrata than neutrophils. Most of the adherent neutrophils were removed during the first 5 minutes incubation in 3 mM EDTA, thereafter, complete detachment of macrophages followed. Neutrophils and macrophages were enriched more than 80% in nonadherent cell populations and detached cell populations, respectively. These separated colostral leukocytes retained their viability and phagocytic activities. Therefore, functional studies of these purified human colostral leukocytes are possible." @default.
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- W2051342615 date "1983-01-01" @default.
- W2051342615 modified "2023-09-23" @default.
- W2051342615 title "Separation of human colostral macrophages and neutrophils on gelatin and collagen-serum substrata." @default.
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- W2051342615 doi "https://doi.org/10.1247/csf.8.367" @default.
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