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- W2051346115 abstract "Trichoderma asperellum SKT-1 and Gibberella fujikuroi, known as causal agents of “Bakanae” disease, were both transformed with genes encoding green fluorescent protein (GFP) and hygromycin B (hygB) by restriction enzyme- mediated integration (REMI). Rice seeds inoculated with GFP-tagged G. fujikuroi showed “Bakanae” symptoms. GFP-tagged SKT-1 maintained biocontrol activity against the pathogen by soaking seeds in SKT-1 spore suspension. Then, we monitored in situ interactions between SKT-1 and G. fujikuroi on rice seeds using GFP-tagged transformations under confocal scanning laser stereomicroscopy. G. fujikuroi disappeared from the embryo of rice seeds after treatment with SKT-1, whereas SKT-1 was observed on the embryo 24 hr after initiation of germination. In addition, the hyphae of G. fujikuroi were penetrated by the hyphae of SKT-1, and degradation of the cell walls of G. fujikuroi was observed under SEM in co-culture. The cell wall of G. fujikuroi on the embryo of rice seeds was lysed, suggesting that mycoparasitism is the mode of action of T. asperellum SKT-1." @default.
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- W2051346115 date "2007-01-01" @default.
- W2051346115 modified "2023-10-14" @default.
- W2051346115 title "Mode of action of Trichoderma asperellum SKT-1, a biocontrol agent against Gibberella fujikuroi" @default.
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- W2051346115 doi "https://doi.org/10.1584/jpestics.g06-35" @default.
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