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- W2051361375 abstract "Claims that low levels of He-Ne laser light (cw, (lambda) equals 632.8 nm) can provide clinical benefits and can enhance in vitro cellular growth are still controversial (T.I. Quickenden and L.L. Daniels, 1993, Photochem. Photobiol. 57, 272-278; L.L. Daniels and T.I. Quickenden, 1994, Photochem. Photobiol., 60, 481-485). The present study tests the suggestion (T.I. Karu, 1988, Lasers life Sci. 2, 53-74; H. Friedmann, R. Lubart, I. Laulicht and S. Rochkink, 1991, J. Photochem. Photobiol. B: Biol. 11, 87-91) that red light stimulates mitosis by raising intracellular pH via absorption by chromophores in the respiratory chain. In order to search for photoinduced changes in intracellular pH, the effect of 5 mW He-Ne laser irradiation on cultures of E. coli was examined using a 300 MHz Nuclear Magnetic Resonance (NMR) spectrometer. The pH difference between the intracellular and extracellular fluid was monitored in the presence and absence of radiation by determining the difference in chemical shift for 31 P resonances arising from the H 2 PO 4 - ⇔ HPO 4 2- + H + equilibrium in the two environments." @default.
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- W2051361375 date "1995-05-22" @default.
- W2051361375 modified "2023-09-23" @default.
- W2051361375 title "Does low intensity He-Ne laser radiation affect the intracellular pH of intact Escherichia coli cells?" @default.
- W2051361375 doi "https://doi.org/10.1117/12.209924" @default.
- W2051361375 hasPublicationYear "1995" @default.
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