FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051419120> ?p ?o ?g. }

• W2051419120 abstract "Abstract Background The foamy virus (FV) replication cycle displays several unique features, which set them apart from orthoretroviruses. First, like other B/D type orthoretroviruses, FV capsids preassemble at the centrosome, but more similar to hepadnaviruses, FV budding is strictly dependent on cognate viral glycoprotein coexpression. Second, the unusually broad host range of FV is thought to be due to use of a very common entry receptor present on host cell plasma membranes, because all cell lines tested in vitro so far are permissive. Results In order to take advantage of modern fluorescent microscopy techniques to study FV replication, we have created FV Gag proteins bearing a variety of protein tags and evaluated these for their ability to support various steps of FV replication. Addition of even small N-terminal HA-tags to FV Gag severely impaired FV particle release. For example, release was completely abrogated by an N-terminal autofluorescent protein (AFP) fusion, despite apparently normal intracellular capsid assembly. In contrast, C-terminal Gag-tags had only minor effects on particle assembly, egress and particle morphogenesis. The infectivity of C-terminal capsid-tagged FV vector particles was reduced up to 100-fold in comparison to wild type; however, infectivity was rescued by coexpression of wild type Gag and assembly of mixed particles. Specific dose-dependent binding of fluorescent FV particles to target cells was demonstrated in an Env-dependent manner, but not binding to target cell-extracted- or synthetic- lipids. Screening of target cells of various origins resulted in the identification of two cell lines, a human erythroid precursor- and a zebrafish- cell line, resistant to FV Env-mediated FV- and HIV-vector transduction. Conclusions We have established functional, autofluorescent foamy viral particles as a valuable new tool to study FV - host cell interactions using modern fluorescent imaging techniques. Furthermore, we succeeded for the first time in identifying two cell lines resistant to Prototype Foamy Virus Env-mediated gene transfer. Interestingly, both cell lines still displayed FV Env-dependent attachment of fluorescent retroviral particles, implying a post-binding block potentially due to lack of putative FV entry cofactors. These cell lines might ultimately lead to the identification of the currently unknown ubiquitous cellular entry receptor(s) of FVs." @default.
• W2051419120 created "2016-06-24" @default.
• W2051419120 creator A5003432938 @default.
• W2051419120 creator A5006337467 @default.
• W2051419120 creator A5009066461 @default.
• W2051419120 creator A5009215021 @default.
• W2051419120 creator A5017322730 @default.
• W2051419120 creator A5029634109 @default.
• W2051419120 creator A5033736706 @default.
• W2051419120 creator A5039731610 @default.
• W2051419120 creator A5043126037 @default.
• W2051419120 creator A5050624967 @default.
• W2051419120 creator A5054291480 @default.
• W2051419120 creator A5059732456 @default.
• W2051419120 creator A5070931387 @default.
• W2051419120 creator A5086296055 @default.
• W2051419120 date "2010-05-17" @default.
• W2051419120 modified "2023-09-27" @default.
• W2051419120 title "Analysis of Prototype Foamy Virus particle-host cell interaction with autofluorescent retroviral particles" @default.
• W2051419120 cites W1034217151 @default.
• W2051419120 cites W1556193709 @default.
• W2051419120 cites W1562103219 @default.
• W2051419120 cites W1568579276 @default.
• W2051419120 cites W1673076679 @default.
• W2051419120 cites W1766420989 @default.
• W2051419120 cites W1905880865 @default.
• W2051419120 cites W1935340730 @default.
• W2051419120 cites W1965156920 @default.
• W2051419120 cites W1968883761 @default.
• W2051419120 cites W1991823169 @default.
• W2051419120 cites W2011996850 @default.
• W2051419120 cites W2023528999 @default.
• W2051419120 cites W2044315573 @default.
• W2051419120 cites W2049509982 @default.
• W2051419120 cites W2051695714 @default.
• W2051419120 cites W2080980712 @default.
• W2051419120 cites W2083517198 @default.
• W2051419120 cites W2091477263 @default.
• W2051419120 cites W2093041047 @default.
• W2051419120 cites W2095760113 @default.
• W2051419120 cites W2098447112 @default.
• W2051419120 cites W2100248438 @default.
• W2051419120 cites W2100368663 @default.
• W2051419120 cites W2101649517 @default.
• W2051419120 cites W2106736422 @default.
• W2051419120 cites W2107913200 @default.
• W2051419120 cites W2108718984 @default.
• W2051419120 cites W2116929075 @default.
• W2051419120 cites W2118990896 @default.
• W2051419120 cites W2122432632 @default.
• W2051419120 cites W2124259497 @default.
• W2051419120 cites W2126631403 @default.
• W2051419120 cites W2128558619 @default.
• W2051419120 cites W2130357985 @default.
• W2051419120 cites W2133389565 @default.
• W2051419120 cites W2133396499 @default.
• W2051419120 cites W2136176308 @default.
• W2051419120 cites W2136330246 @default.
• W2051419120 cites W2138427302 @default.
• W2051419120 cites W2143140715 @default.
• W2051419120 cites W2145860891 @default.
• W2051419120 cites W2147157181 @default.
• W2051419120 cites W2148335667 @default.
• W2051419120 cites W2148522104 @default.
• W2051419120 cites W2152451108 @default.
• W2051419120 cites W2154810129 @default.
• W2051419120 cites W2157309681 @default.
• W2051419120 cites W2160726747 @default.
• W2051419120 cites W2165745296 @default.
• W2051419120 cites W2165918134 @default.
• W2051419120 cites W2166932109 @default.
• W2051419120 cites W2168526937 @default.
• W2051419120 cites W2401811518 @default.
• W2051419120 cites W4234590243 @default.
• W2051419120 doi "https://doi.org/10.1186/1742-4690-7-45" @default.
• W2051419120 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2887381" @default.
• W2051419120 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20478027" @default.
• W2051419120 hasPublicationYear "2010" @default.
• W2051419120 type Work @default.
• W2051419120 sameAs 2051419120 @default.
• W2051419120 citedByCount "60" @default.
• W2051419120 countsByYear W20514191202012 @default.
• W2051419120 countsByYear W20514191202013 @default.
• W2051419120 countsByYear W20514191202014 @default.
• W2051419120 countsByYear W20514191202015 @default.
• W2051419120 countsByYear W20514191202016 @default.
• W2051419120 countsByYear W20514191202017 @default.
• W2051419120 countsByYear W20514191202018 @default.
• W2051419120 countsByYear W20514191202019 @default.
• W2051419120 countsByYear W20514191202020 @default.
• W2051419120 countsByYear W20514191202021 @default.
• W2051419120 countsByYear W20514191202022 @default.
• W2051419120 countsByYear W20514191202023 @default.
• W2051419120 crossrefType "journal-article" @default.
• W2051419120 hasAuthorship W2051419120A5003432938 @default.
• W2051419120 hasAuthorship W2051419120A5006337467 @default.
• W2051419120 hasAuthorship W2051419120A5009066461 @default.
• W2051419120 hasAuthorship W2051419120A5009215021 @default.
• W2051419120 hasAuthorship W2051419120A5017322730 @default.
• W2051419120 hasAuthorship W2051419120A5029634109 @default.

• next