Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051441091> ?p ?o ?g. }
- W2051441091 endingPage "1035" @default.
- W2051441091 startingPage "1019" @default.
- W2051441091 abstract "A single-shot Hepatitis B vaccine formulation using poly(d,l)-lactide-co-glycolide acid (PLGA) microspheres as a delivery system was examined using a variety of biophysical and biochemical techniques as well as immunological evaluation in C3H mice. PLGA microsphere encapsulation of the Hepatitis B surface antigen (HBsAg), a lipoprotein particle, resulted in good recoveries of protein mass, protein particle conformational integrity, and in vitro antigenicity. Some partial delipidation of the HBsAg, however, was observed. The loading and encapsulation efficiency of HBsAg into the PLGA microspheres were measured along with the morphology and size distribution of the vaccine-loaded PLGA microspheres. The in vitro release kinetics of HBsAg from the PLGA microspheres was evaluated and found to be affected by experimental conditions such as stirring rate. HBsAg showed enhanced storage stability at 37 degrees C in the slightly acidic pH range reported to be found inside PLGA microspheres; thus, the antigen is relatively stable under conditions of temperature and pH that may mimic in vivo conditions. The immunogenicity of the microsphere formulations of HBsAg was compared with conventional aluminum adjuvant formulated HBsAg vaccine in C3H mice. Comparisons were made between aluminum formulations (one and two injections), PLGA microsphere formulations (single injection), and a mixture of aluminum and PLGA microsphere formulations (single injection). The nine-month serum antibody titers indicate that a single injection of a mixture of aluminum and PLGA-formulated HBsAg results in equal or better immune responses than two injections of aluminum-formulated HBsAg vaccine. Based on these in vitro and in vivo studies, it is concluded that HBsAg can be successfully encapsulated and recovered from the PLGA microspheres and a mixture of aluminum-adjuvanted and PLGA-formulated HBsAg can auto-boost an immune response in manner comparable to multiple injections of an aluminum-formulated vaccine." @default.
- W2051441091 created "2016-06-24" @default.
- W2051441091 creator A5013351347 @default.
- W2051441091 creator A5014345880 @default.
- W2051441091 creator A5026833623 @default.
- W2051441091 creator A5068447468 @default.
- W2051441091 creator A5071125700 @default.
- W2051441091 creator A5080198382 @default.
- W2051441091 date "2002-04-01" @default.
- W2051441091 modified "2023-09-24" @default.
- W2051441091 title "Pharmaceutical and Immunological Evaluation of a Single-Shot Hepatitis B Vaccine Formulated With PLGA Microspheres" @default.
- W2051441091 cites W105445468 @default.
- W2051441091 cites W118779968 @default.
- W2051441091 cites W1490430764 @default.
- W2051441091 cites W1510773961 @default.
- W2051441091 cites W1526203517 @default.
- W2051441091 cites W1547433582 @default.
- W2051441091 cites W1576437441 @default.
- W2051441091 cites W1632470944 @default.
- W2051441091 cites W1964807748 @default.
- W2051441091 cites W1965163412 @default.
- W2051441091 cites W1971187651 @default.
- W2051441091 cites W1980073782 @default.
- W2051441091 cites W1982201600 @default.
- W2051441091 cites W1985882639 @default.
- W2051441091 cites W1986159473 @default.
- W2051441091 cites W1989641932 @default.
- W2051441091 cites W1990073207 @default.
- W2051441091 cites W1993135217 @default.
- W2051441091 cites W1995941499 @default.
- W2051441091 cites W1997899946 @default.
- W2051441091 cites W2000998234 @default.
- W2051441091 cites W2005950750 @default.
- W2051441091 cites W2017145464 @default.
- W2051441091 cites W2020355224 @default.
- W2051441091 cites W2020386751 @default.
- W2051441091 cites W2024226059 @default.
- W2051441091 cites W2031079435 @default.
- W2051441091 cites W2031297642 @default.
- W2051441091 cites W2032247822 @default.
- W2051441091 cites W2032888813 @default.
- W2051441091 cites W2033014239 @default.
- W2051441091 cites W2033852067 @default.
- W2051441091 cites W2034194003 @default.
- W2051441091 cites W2035953055 @default.
- W2051441091 cites W2036550525 @default.
- W2051441091 cites W2037293465 @default.
- W2051441091 cites W2037311180 @default.
- W2051441091 cites W2042405882 @default.
- W2051441091 cites W2058416686 @default.
- W2051441091 cites W2059058735 @default.
- W2051441091 cites W2061009669 @default.
- W2051441091 cites W2063092454 @default.
- W2051441091 cites W2064579077 @default.
- W2051441091 cites W2074061676 @default.
- W2051441091 cites W2077562656 @default.
- W2051441091 cites W2085714772 @default.
- W2051441091 cites W2085731798 @default.
- W2051441091 cites W2095438503 @default.
- W2051441091 cites W2104890606 @default.
- W2051441091 cites W2105293363 @default.
- W2051441091 cites W2106641996 @default.
- W2051441091 cites W2110416036 @default.
- W2051441091 cites W2139324621 @default.
- W2051441091 cites W213967698 @default.
- W2051441091 cites W2147904584 @default.
- W2051441091 cites W2152758574 @default.
- W2051441091 cites W2155274524 @default.
- W2051441091 cites W2167126715 @default.
- W2051441091 cites W2176182974 @default.
- W2051441091 cites W2278233976 @default.
- W2051441091 cites W231269100 @default.
- W2051441091 cites W283469004 @default.
- W2051441091 cites W3216583532 @default.
- W2051441091 cites W76994341 @default.
- W2051441091 cites W85159053 @default.
- W2051441091 doi "https://doi.org/10.1002/jps.10042" @default.
- W2051441091 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11948541" @default.
- W2051441091 hasPublicationYear "2002" @default.
- W2051441091 type Work @default.
- W2051441091 sameAs 2051441091 @default.
- W2051441091 citedByCount "90" @default.
- W2051441091 countsByYear W20514410912012 @default.
- W2051441091 countsByYear W20514410912013 @default.
- W2051441091 countsByYear W20514410912014 @default.
- W2051441091 countsByYear W20514410912015 @default.
- W2051441091 countsByYear W20514410912016 @default.
- W2051441091 countsByYear W20514410912017 @default.
- W2051441091 countsByYear W20514410912018 @default.
- W2051441091 countsByYear W20514410912019 @default.
- W2051441091 countsByYear W20514410912020 @default.
- W2051441091 countsByYear W20514410912021 @default.
- W2051441091 countsByYear W20514410912022 @default.
- W2051441091 crossrefType "journal-article" @default.
- W2051441091 hasAuthorship W2051441091A5013351347 @default.
- W2051441091 hasAuthorship W2051441091A5014345880 @default.
- W2051441091 hasAuthorship W2051441091A5026833623 @default.
- W2051441091 hasAuthorship W2051441091A5068447468 @default.