FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051448427> ?p ?o ?g. }

• W2051448427 endingPage "3130" @default.
• W2051448427 startingPage "3130" @default.
• W2051448427 abstract "We report a mechanistic study of how flow and recirculation in a microreactor can be used to optimize the capture and release of stimuli-responsive polymer-protein reagents on stimuli-responsive polymer-grafted channel surfaces. Poly(N-isopropylacrylamide) (PNIPAAm) was grafted to polydimethylsiloxane (PDMS) channel walls, creating switchable surfaces where PNIPAAm-protein conjugates would adhere at temperatures above the lower critical solution temperature (LCST) and released below the LCST. A PNIPAAm-streptavidin conjugate that can capture biotinylated antibody-antigen targets was first characterized. The conjugate's immobilization and release were limited by mass transport to and from the functionalized PNIPAAm surface. Transport and adsorption efficiencies were dependent on the aggregate size of the PNIPAAm-streptavidin conjugate above the LCST and also were dependent on whether the conjugates were heated in the presence of the stimuli-responsive surface or pre-aggregated and then flowed across the surface. As conjugate size increased, through the addition of non-conjugated PNIPAAm, recirculation and mixing were shown to markedly improve conjugate immobilization compared to diffusion alone. Under optimized conditions of flow and reagent concentrations, approximately 60% of the streptavidin conjugate bolus could be captured at the surface and subsequently successfully released. The kinetic release profile sharpness was also strongly improved with recirculation and helical mixing. Finally, the concentration of protein-polymer conjugates could be achieved by continuous conjugate flow into the heated recirculator, allowing nearly linear enrichment of the conjugate reagent from larger volumes. This capability was shown with anti-p24 HIV monoclonal antibody reagents that were enriched over 5-fold using this protocol. These studies provide insight into the mechanism of smart polymer-protein conjugate capture and release in grafted channels and show the potential of this purification and enrichment module for processing diagnostic samples." @default.
• W2051448427 created "2016-06-24" @default.
• W2051448427 creator A5008596372 @default.
• W2051448427 creator A5049288421 @default.
• W2051448427 creator A5049846376 @default.
• W2051448427 creator A5058761588 @default.
• W2051448427 creator A5064390595 @default.
• W2051448427 creator A5068734484 @default.
• W2051448427 date "2010-01-01" @default.
• W2051448427 modified "2023-10-16" @default.
• W2051448427 title "A helical flow, circular microreactor for separating and enriching “smart” polymer–antibody capture reagents" @default.
• W2051448427 cites W1953822958 @default.
• W2051448427 cites W1974677682 @default.
• W2051448427 cites W1978584972 @default.
• W2051448427 cites W1985800449 @default.
• W2051448427 cites W1991883036 @default.
• W2051448427 cites W1993465142 @default.
• W2051448427 cites W1993504981 @default.
• W2051448427 cites W1994846754 @default.
• W2051448427 cites W1996294361 @default.
• W2051448427 cites W1998920415 @default.
• W2051448427 cites W1999785107 @default.
• W2051448427 cites W2000884295 @default.
• W2051448427 cites W2001544196 @default.
• W2051448427 cites W2008046073 @default.
• W2051448427 cites W2010958193 @default.
• W2051448427 cites W2015086447 @default.
• W2051448427 cites W2021337135 @default.
• W2051448427 cites W2022520585 @default.
• W2051448427 cites W2023956722 @default.
• W2051448427 cites W2026087250 @default.
• W2051448427 cites W2029433156 @default.
• W2051448427 cites W2030569221 @default.
• W2051448427 cites W2037269788 @default.
• W2051448427 cites W2038444472 @default.
• W2051448427 cites W2046162071 @default.
• W2051448427 cites W2047943947 @default.
• W2051448427 cites W2048197073 @default.
• W2051448427 cites W2049638225 @default.
• W2051448427 cites W2056395418 @default.
• W2051448427 cites W2064243806 @default.
• W2051448427 cites W2069570927 @default.
• W2051448427 cites W2070378283 @default.
• W2051448427 cites W2070770020 @default.
• W2051448427 cites W2071061339 @default.
• W2051448427 cites W2071311642 @default.
• W2051448427 cites W2077584139 @default.
• W2051448427 cites W2079745449 @default.
• W2051448427 cites W2090787374 @default.
• W2051448427 cites W2090803851 @default.
• W2051448427 cites W2092511947 @default.
• W2051448427 cites W2105577215 @default.
• W2051448427 cites W2105963254 @default.
• W2051448427 cites W2115240308 @default.
• W2051448427 cites W2116208923 @default.
• W2051448427 cites W2128609649 @default.
• W2051448427 cites W2139169403 @default.
• W2051448427 cites W2150116877 @default.
• W2051448427 cites W2159606304 @default.
• W2051448427 cites W2166213615 @default.
• W2051448427 cites W2167977108 @default.
• W2051448427 cites W2170139878 @default.
• W2051448427 cites W2172116519 @default.
• W2051448427 doi "https://doi.org/10.1039/c004978f" @default.
• W2051448427 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3116725" @default.
• W2051448427 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20882219" @default.
• W2051448427 hasPublicationYear "2010" @default.
• W2051448427 type Work @default.
• W2051448427 sameAs 2051448427 @default.
• W2051448427 citedByCount "29" @default.
• W2051448427 countsByYear W20514484272012 @default.
• W2051448427 countsByYear W20514484272013 @default.
• W2051448427 countsByYear W20514484272014 @default.
• W2051448427 countsByYear W20514484272016 @default.
• W2051448427 countsByYear W20514484272017 @default.
• W2051448427 countsByYear W20514484272019 @default.
• W2051448427 countsByYear W20514484272020 @default.
• W2051448427 countsByYear W20514484272021 @default.
• W2051448427 countsByYear W20514484272022 @default.
• W2051448427 countsByYear W20514484272023 @default.
• W2051448427 crossrefType "journal-article" @default.
• W2051448427 hasAuthorship W2051448427A5008596372 @default.
• W2051448427 hasAuthorship W2051448427A5049288421 @default.
• W2051448427 hasAuthorship W2051448427A5049846376 @default.
• W2051448427 hasAuthorship W2051448427A5058761588 @default.
• W2051448427 hasAuthorship W2051448427A5064390595 @default.
• W2051448427 hasAuthorship W2051448427A5068734484 @default.
• W2051448427 hasBestOaLocation W20514484272 @default.
• W2051448427 hasConcept C127413603 @default.
• W2051448427 hasConcept C134306372 @default.
• W2051448427 hasConcept C147831808 @default.
• W2051448427 hasConcept C150394285 @default.
• W2051448427 hasConcept C15920480 @default.
• W2051448427 hasConcept C161790260 @default.
• W2051448427 hasConcept C178790620 @default.
• W2051448427 hasConcept C185592680 @default.
• W2051448427 hasConcept C188109995 @default.
• W2051448427 hasConcept C192562407 @default.

• next