FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051489680> ?p ?o ?g. }

• W2051489680 abstract "Abstract Background Small non-coding RNAs (ncRNAs) are important regulators of gene expression in eukaryotes. Previously, only microRNAs (miRNAs) and piRNAs have been identified in the silkworm, Bombyx mori . Furthermore, only ncRNAs (50-500nt) of intermediate size have been systematically identified in the silkworm. Results Here, we performed a systematic identification and analysis of small RNAs (18-50nt) associated with the Bombyx mori argonaute2 (BmAgo2) protein. Using RIP-seq, we identified various types of small ncRNAs associated with BmAGO2. These ncRNAs showed a multimodal length distribution, with three peaks at ~20nt, ~27nt and ~33nt, which included tRNA-, transposable element (TE)-, rRNA-, snoRNA- and snRNA-derived small RNAs as well as miRNAs and piRNAs. The tRNA-derived fragments (tRFs) were found at an extremely high abundance and accounted for 69.90% of the BmAgo2-associated small RNAs. Northern blotting confirmed that many tRFs were expressed or up-regulated only in the BmNPV-infected cells, implying that the tRFs play a prominent role by binding to BmAgo2 during BmNPV infection. Additional evidence suggested that there are potential cleavage sites on the D, anti-codon and TψC loops of the tRNAs. TE-derived small RNAs and piRNAs also accounted for a significant proportion of the BmAgo2-associated small RNAs, suggesting that BmAgo2 could be involved in the maintenance of genome stability by suppressing the activities of transposons guided by these small RNAs. Finally, Northern blotting was also used to confirm the Bombyx 5.8 s rRNA-derived small RNAs, demonstrating that various novel small RNAs exist in the silkworm. Conclusions Using an RIP-seq method in combination with Northern blotting, we identified various types of small RNAs associated with the BmAgo2 protein, including tRNA-, TE-, rRNA-, snoRNA- and snRNA-derived small RNAs as well as miRNAs and piRNAs. Our findings provide new clues for future functional studies of the role of small RNAs in insect development and evolution." @default.
• W2051489680 created "2016-06-24" @default.
• W2051489680 creator A5001937247 @default.
• W2051489680 creator A5002607553 @default.
• W2051489680 creator A5011297387 @default.
• W2051489680 creator A5032958129 @default.
• W2051489680 creator A5035333148 @default.
• W2051489680 creator A5036066093 @default.
• W2051489680 creator A5038851564 @default.
• W2051489680 creator A5044225148 @default.
• W2051489680 creator A5045197865 @default.
• W2051489680 creator A5050766425 @default.
• W2051489680 creator A5067459123 @default.
• W2051489680 creator A5068349871 @default.
• W2051489680 creator A5069770938 @default.
• W2051489680 creator A5078126584 @default.
• W2051489680 creator A5088299761 @default.
• W2051489680 date "2013-09-28" @default.
• W2051489680 modified "2023-10-16" @default.
• W2051489680 title "RIP-seq of BmAgo2-associated small RNAs reveal various types of small non-coding RNAs in the silkworm, Bombyx mori" @default.
• W2051489680 cites W144423133 @default.
• W2051489680 cites W1498663519 @default.
• W2051489680 cites W1560642816 @default.
• W2051489680 cites W1601491244 @default.
• W2051489680 cites W180794048 @default.
• W2051489680 cites W1967884424 @default.
• W2051489680 cites W1968008181 @default.
• W2051489680 cites W1973726689 @default.
• W2051489680 cites W1975437957 @default.
• W2051489680 cites W1976355133 @default.
• W2051489680 cites W1984771273 @default.
• W2051489680 cites W1985480639 @default.
• W2051489680 cites W1987067847 @default.
• W2051489680 cites W1988717422 @default.
• W2051489680 cites W1990061381 @default.
• W2051489680 cites W1990231660 @default.
• W2051489680 cites W1991007428 @default.
• W2051489680 cites W1997434013 @default.
• W2051489680 cites W1997591982 @default.
• W2051489680 cites W2000210567 @default.
• W2051489680 cites W2004378660 @default.
• W2051489680 cites W2007995608 @default.
• W2051489680 cites W2010890469 @default.
• W2051489680 cites W2018989934 @default.
• W2051489680 cites W2024829095 @default.
• W2051489680 cites W2026257049 @default.
• W2051489680 cites W2026685039 @default.
• W2051489680 cites W2028772621 @default.
• W2051489680 cites W2030246519 @default.
• W2051489680 cites W2030676673 @default.
• W2051489680 cites W2033724104 @default.
• W2051489680 cites W2035340311 @default.
• W2051489680 cites W2038416631 @default.
• W2051489680 cites W2038521536 @default.
• W2051489680 cites W2040058302 @default.
• W2051489680 cites W2046109186 @default.
• W2051489680 cites W2046214303 @default.
• W2051489680 cites W2050878024 @default.
• W2051489680 cites W2054044390 @default.
• W2051489680 cites W2058538156 @default.
• W2051489680 cites W2058945550 @default.
• W2051489680 cites W2059357290 @default.
• W2051489680 cites W2065416213 @default.
• W2051489680 cites W2069354765 @default.
• W2051489680 cites W2069408870 @default.
• W2051489680 cites W2072972020 @default.
• W2051489680 cites W2073875157 @default.
• W2051489680 cites W2074106269 @default.
• W2051489680 cites W2074216902 @default.
• W2051489680 cites W2074471151 @default.
• W2051489680 cites W2074811101 @default.
• W2051489680 cites W2077746133 @default.
• W2051489680 cites W2078491728 @default.
• W2051489680 cites W2078608650 @default.
• W2051489680 cites W2080233931 @default.
• W2051489680 cites W2085841113 @default.
• W2051489680 cites W2086848163 @default.
• W2051489680 cites W2087463572 @default.
• W2051489680 cites W2095238116 @default.
• W2051489680 cites W2095479321 @default.
• W2051489680 cites W2097341408 @default.
• W2051489680 cites W2097421126 @default.
• W2051489680 cites W2097606916 @default.
• W2051489680 cites W2101141163 @default.
• W2051489680 cites W2103944505 @default.
• W2051489680 cites W2104582743 @default.
• W2051489680 cites W2106888954 @default.
• W2051489680 cites W2107017930 @default.
• W2051489680 cites W2107327538 @default.
• W2051489680 cites W2109140791 @default.
• W2051489680 cites W2112732396 @default.
• W2051489680 cites W2119962312 @default.
• W2051489680 cites W2121196786 @default.
• W2051489680 cites W2124985265 @default.
• W2051489680 cites W2125346459 @default.
• W2051489680 cites W2128746033 @default.
• W2051489680 cites W2132077305 @default.
• W2051489680 cites W2139871850 @default.
• W2051489680 cites W2142754990 @default.
• W2051489680 cites W2144490750 @default.

• next