FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051504182> ?p ?o ?g. }

• W2051504182 endingPage "2556" @default.
• W2051504182 startingPage "2549" @default.
• W2051504182 abstract "Forensic and ancient DNA (aDNA) extracts are mixtures of endogenous aDNA, existing in more or less damaged state, and contaminant DNA. To obtain the true aDNA sequence, it is not sufficient to generate a single direct sequence of the mixture, even where the authentic aDNA is the most abundant (e.g. 25% or more) in the component mixture. Only bacterial cloning can elucidate the components of this mixture. We calculate the number of clones that need to be sampled (for various mixture ratios) in order to be confident (at various levels of confidence) to have identified the major component. We demonstrate that to be >95% confident of identifying the most abundant sequence present at 70% in the ancient sample, 20 clones must be sampled. We make recommendations and offer a free-access web-based program, which constructs the most reliable consensus sequence from the user's input clone sequences and analyses the confidence limits for each nucleotide position and for the whole consensus sequence. Accepted authentication methods must be employed in order to assess the authenticity and endogeneity of the resulting consensus sequences (e.g. quantification and replication by another laboratory, blind testing, amelogenin sex versus morphological sex, the effective use of controls, etc.) and determine whether they are indeed aDNA." @default.
• W2051504182 created "2016-06-24" @default.
• W2051504182 creator A5002485044 @default.
• W2051504182 creator A5012022428 @default.
• W2051504182 creator A5030809788 @default.
• W2051504182 creator A5044532377 @default.
• W2051504182 creator A5081686489 @default.
• W2051504182 date "2005-04-28" @default.
• W2051504182 modified "2023-09-28" @default.
• W2051504182 title "How many clones need to be sequenced from a single forensic or ancient DNA sample in order to determine a reliable consensus sequence?" @default.
• W2051504182 cites W1489068731 @default.
• W2051504182 cites W1550571154 @default.
• W2051504182 cites W1579501135 @default.
• W2051504182 cites W1970142955 @default.
• W2051504182 cites W1973214876 @default.
• W2051504182 cites W1982899758 @default.
• W2051504182 cites W2013367562 @default.
• W2051504182 cites W2014081501 @default.
• W2051504182 cites W2015422125 @default.
• W2051504182 cites W2027615939 @default.
• W2051504182 cites W2029644208 @default.
• W2051504182 cites W2030518716 @default.
• W2051504182 cites W2036749277 @default.
• W2051504182 cites W2039208027 @default.
• W2051504182 cites W2047825885 @default.
• W2051504182 cites W2048555786 @default.
• W2051504182 cites W2057077804 @default.
• W2051504182 cites W2058101978 @default.
• W2051504182 cites W2081652203 @default.
• W2051504182 cites W2087842506 @default.
• W2051504182 cites W2107040819 @default.
• W2051504182 cites W2109291482 @default.
• W2051504182 cites W2127075404 @default.
• W2051504182 cites W2130460780 @default.
• W2051504182 cites W2139115044 @default.
• W2051504182 cites W2151920427 @default.
• W2051504182 cites W2153930709 @default.
• W2051504182 cites W2155058110 @default.
• W2051504182 doi "https://doi.org/10.1093/nar/gki550" @default.
• W2051504182 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1088305" @default.
• W2051504182 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15870386" @default.
• W2051504182 hasPublicationYear "2005" @default.
• W2051504182 type Work @default.
• W2051504182 sameAs 2051504182 @default.
• W2051504182 citedByCount "44" @default.
• W2051504182 countsByYear W20515041822012 @default.
• W2051504182 countsByYear W20515041822013 @default.
• W2051504182 countsByYear W20515041822014 @default.
• W2051504182 countsByYear W20515041822015 @default.
• W2051504182 countsByYear W20515041822016 @default.
• W2051504182 crossrefType "journal-article" @default.
• W2051504182 hasAuthorship W2051504182A5002485044 @default.
• W2051504182 hasAuthorship W2051504182A5012022428 @default.
• W2051504182 hasAuthorship W2051504182A5030809788 @default.
• W2051504182 hasAuthorship W2051504182A5044532377 @default.
• W2051504182 hasAuthorship W2051504182A5081686489 @default.
• W2051504182 hasBestOaLocation W20515041822 @default.
• W2051504182 hasConcept C104317684 @default.
• W2051504182 hasConcept C106434640 @default.
• W2051504182 hasConcept C135763542 @default.
• W2051504182 hasConcept C144024400 @default.
• W2051504182 hasConcept C149923435 @default.
• W2051504182 hasConcept C189338579 @default.
• W2051504182 hasConcept C2778112365 @default.
• W2051504182 hasConcept C2908647359 @default.
• W2051504182 hasConcept C3017666073 @default.
• W2051504182 hasConcept C51679486 @default.
• W2051504182 hasConcept C54355233 @default.
• W2051504182 hasConcept C54985914 @default.
• W2051504182 hasConcept C552990157 @default.
• W2051504182 hasConcept C70721500 @default.
• W2051504182 hasConcept C77434933 @default.
• W2051504182 hasConcept C86803240 @default.
• W2051504182 hasConceptScore W2051504182C104317684 @default.
• W2051504182 hasConceptScore W2051504182C106434640 @default.
• W2051504182 hasConceptScore W2051504182C135763542 @default.
• W2051504182 hasConceptScore W2051504182C144024400 @default.
• W2051504182 hasConceptScore W2051504182C149923435 @default.
• W2051504182 hasConceptScore W2051504182C189338579 @default.
• W2051504182 hasConceptScore W2051504182C2778112365 @default.
• W2051504182 hasConceptScore W2051504182C2908647359 @default.
• W2051504182 hasConceptScore W2051504182C3017666073 @default.
• W2051504182 hasConceptScore W2051504182C51679486 @default.
• W2051504182 hasConceptScore W2051504182C54355233 @default.
• W2051504182 hasConceptScore W2051504182C54985914 @default.
• W2051504182 hasConceptScore W2051504182C552990157 @default.
• W2051504182 hasConceptScore W2051504182C70721500 @default.
• W2051504182 hasConceptScore W2051504182C77434933 @default.
• W2051504182 hasConceptScore W2051504182C86803240 @default.
• W2051504182 hasIssue "8" @default.
• W2051504182 hasLocation W20515041821 @default.
• W2051504182 hasLocation W20515041822 @default.
• W2051504182 hasLocation W20515041823 @default.
• W2051504182 hasLocation W20515041824 @default.
• W2051504182 hasOpenAccess W2051504182 @default.
• W2051504182 hasPrimaryLocation W20515041821 @default.
• W2051504182 hasRelatedWork W2003848246 @default.
• W2051504182 hasRelatedWork W2014681313 @default.

• next