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• W2051507265 abstract "The pathway of dephosphorylation of myo-inositol hexakisphosphate by the phytate-degrading enzymes of Bacillus subtilis 168, Bacillus amyloliquefaciens ATCC 15841, and Bacillus amyloliquefaciens 45 was established using a combination of high-performance ion chromatography analysis and kinetic studies. The data demonstrate that all the Bacillus phytate-degrading enzymes under investigation dephosphorylate myo-inositol hexakisphosphate by sequential removal of phosphate groups via two independent routes; the routes proceed via D-Ins(1,2,4,5,6)P 5 to Ins(2,4,5,6)P 4 to finally Ins(2,4,6)P 3 or D-Ins(2,5,6)P 3 and via D-Ins(1,2,4,5,6)P 5 to D-Ins(1,2,5,6)P 4 to finally D-Ins(1,2,6)P 3 . The resulting myo-inositol trisphosphate D-Ins(1,2,6)P 3 was degraded via D-Ins(2,6)P 2 to finally Ins(2)P after prolonged incubation times in combination with increased enzyme concentration. Key words: Bacillus spp., myo-inositol phosphate isomers, phytase, phytate degradation." @default.
• W2051507265 created "2016-06-24" @default.
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• W2051507265 date "2002-11-01" @default.
• W2051507265 modified "2023-10-10" @default.
• W2051507265 title "The pathway of dephosphorylation of <i>myo</i>-inositol hexakisphosphate by phytate-degrading enzymes of different <i>Bacillus </i>spp." @default.
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• W2051507265 doi "https://doi.org/10.1139/w02-097" @default.
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