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• W2051507406 abstract "Abstract Human fetal Purkinje fibres have in the present study for the first time been identified at the ultrastructural level. This has been accomplished by a comparison of light microscopic criteria for identification (high content of the intermediate filament protein skeletin and high cholinesterase activity) with immunohistochemical and ultrastructural observations of the myofibrillar M-band. In sections incubated with antibodies against the M-line protein MM-creatine kinase the Purkinje fibres showed a cross-striated fluorescence pattern, while the ordinary ventricular myocytes did not. Ultrastructurally a subendocardial cell population in the ventricles was distinguished from ordinary myocytes by containing electron dense M-bands. Since MM-creatine kinase is considered to make an essential contribution to the electron density of the M-band we conclude that the ultrastructurally distinguished cell population represents the Purkinje fibres. In some areas of the atria the myofibrils were also labelled by MM-creatine kinase antibodies and contained electron dense M-bands. This indicates that myofibrillar differentiation proceeds independently in different cardiac tissues." @default.
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• W2051507406 date "1982-08-01" @default.
• W2051507406 modified "2023-10-02" @default.
• W2051507406 title "Ultrastructural identification of human fetal Purkinje fibres—a comparative immunocytochemical and electron microscopic study of composition and structure of myofibrillar M-regions" @default.
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• W2051507406 doi "https://doi.org/10.1016/0022-2828(82)90150-x" @default.
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• W2051507406 hasPublicationYear "1982" @default.
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