Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051552213> ?p ?o ?g. }
- W2051552213 endingPage "394" @default.
- W2051552213 startingPage "385" @default.
- W2051552213 abstract "Objective: In this study, we inhibited the expression of VEGF by short hairpin RNA (shRNA) in SKOV3 ovarian cancer cells in vitro and in vivo to explore the antitumor efficacy of shRNA in ovarian cancer cells. Methods: ShRNA targeting VEGF was cloned into pGenesil-2 plasmid vector and then transfected into SKOV3 ovarian cancer cells using liposome. Silencing of VEGF expression was measured by RT-PCR and ELISA assays. Furthermore, the growth inhibition capacity of shRNA on SKOV3 intraperitoneal ovarian carcinomatosis was tested in nude mice. Tumor weight was measured. Microvessel density, number of apoptotic cells and proliferation index in tumor tissues were assessed by CD31, TUNEL and PCNA immunostaining. Results: shRNA targeting VEGF significantly silenced VEGF expression in SKOV3 ovarian cancer cells, confirmed by RT-PCR and ELISA assays (p < 0.05). In vivo, the shRNA reduced tumor weight by ∼60.3% compared with control groups (p < 0.05), accompanied with angiogenesis inhibition (p < 0.01) and apoptosis induction (p < 0.01). Conclusion: Our data indicated that shRNA-mediated silencing of VEGF might be a promising therapeutic strategy against ovarian cancer by reducing angiogenesis and inducing apoptosis." @default.
- W2051552213 created "2016-06-24" @default.
- W2051552213 creator A5000185647 @default.
- W2051552213 creator A5011812917 @default.
- W2051552213 creator A5016604112 @default.
- W2051552213 creator A5021527564 @default.
- W2051552213 creator A5023295164 @default.
- W2051552213 creator A5026804113 @default.
- W2051552213 creator A5029852189 @default.
- W2051552213 creator A5035528148 @default.
- W2051552213 creator A5036757924 @default.
- W2051552213 creator A5045909068 @default.
- W2051552213 creator A5049692788 @default.
- W2051552213 creator A5067258811 @default.
- W2051552213 creator A5074149482 @default.
- W2051552213 creator A5086259408 @default.
- W2051552213 creator A5090145833 @default.
- W2051552213 date "2009-01-01" @default.
- W2051552213 modified "2023-10-14" @default.
- W2051552213 title "VEGF-Targeted Short Hairpin RNA Inhibits Intraperitoneal Ovarian Cancer Growth in Nude Mice" @default.
- W2051552213 cites W1484771785 @default.
- W2051552213 cites W1593130318 @default.
- W2051552213 cites W184581706 @default.
- W2051552213 cites W1977601636 @default.
- W2051552213 cites W1981301410 @default.
- W2051552213 cites W1995483670 @default.
- W2051552213 cites W2002049222 @default.
- W2051552213 cites W2002517161 @default.
- W2051552213 cites W2004860729 @default.
- W2051552213 cites W2009855429 @default.
- W2051552213 cites W2010468452 @default.
- W2051552213 cites W2012063336 @default.
- W2051552213 cites W2018116983 @default.
- W2051552213 cites W2018729653 @default.
- W2051552213 cites W2031229283 @default.
- W2051552213 cites W2033974379 @default.
- W2051552213 cites W2041008224 @default.
- W2051552213 cites W2043103956 @default.
- W2051552213 cites W2052312685 @default.
- W2051552213 cites W2055402663 @default.
- W2051552213 cites W2064726235 @default.
- W2051552213 cites W2089046282 @default.
- W2051552213 cites W2091828677 @default.
- W2051552213 cites W2096663654 @default.
- W2051552213 cites W2102452709 @default.
- W2051552213 cites W2109350154 @default.
- W2051552213 cites W2111435266 @default.
- W2051552213 cites W2130968156 @default.
- W2051552213 cites W2143219457 @default.
- W2051552213 cites W2144959112 @default.
- W2051552213 cites W2146113634 @default.
- W2051552213 cites W2146953551 @default.
- W2051552213 cites W2158743409 @default.
- W2051552213 cites W2163368401 @default.
- W2051552213 cites W2165332575 @default.
- W2051552213 cites W2169880959 @default.
- W2051552213 cites W2170952917 @default.
- W2051552213 cites W2171275402 @default.
- W2051552213 cites W2188274605 @default.
- W2051552213 cites W2223825899 @default.
- W2051552213 cites W2280750701 @default.
- W2051552213 cites W2341149903 @default.
- W2051552213 cites W2412169570 @default.
- W2051552213 doi "https://doi.org/10.1159/000279385" @default.
- W2051552213 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20130421" @default.
- W2051552213 hasPublicationYear "2009" @default.
- W2051552213 type Work @default.
- W2051552213 sameAs 2051552213 @default.
- W2051552213 citedByCount "11" @default.
- W2051552213 countsByYear W20515522132012 @default.
- W2051552213 countsByYear W20515522132013 @default.
- W2051552213 countsByYear W20515522132016 @default.
- W2051552213 countsByYear W20515522132018 @default.
- W2051552213 countsByYear W20515522132019 @default.
- W2051552213 countsByYear W20515522132021 @default.
- W2051552213 crossrefType "journal-article" @default.
- W2051552213 hasAuthorship W2051552213A5000185647 @default.
- W2051552213 hasAuthorship W2051552213A5011812917 @default.
- W2051552213 hasAuthorship W2051552213A5016604112 @default.
- W2051552213 hasAuthorship W2051552213A5021527564 @default.
- W2051552213 hasAuthorship W2051552213A5023295164 @default.
- W2051552213 hasAuthorship W2051552213A5026804113 @default.
- W2051552213 hasAuthorship W2051552213A5029852189 @default.
- W2051552213 hasAuthorship W2051552213A5035528148 @default.
- W2051552213 hasAuthorship W2051552213A5036757924 @default.
- W2051552213 hasAuthorship W2051552213A5045909068 @default.
- W2051552213 hasAuthorship W2051552213A5049692788 @default.
- W2051552213 hasAuthorship W2051552213A5067258811 @default.
- W2051552213 hasAuthorship W2051552213A5074149482 @default.
- W2051552213 hasAuthorship W2051552213A5086259408 @default.
- W2051552213 hasAuthorship W2051552213A5090145833 @default.
- W2051552213 hasConcept C104317684 @default.
- W2051552213 hasConcept C119056186 @default.
- W2051552213 hasConcept C121608353 @default.
- W2051552213 hasConcept C126322002 @default.
- W2051552213 hasConcept C150903083 @default.
- W2051552213 hasConcept C153911025 @default.
- W2051552213 hasConcept C167734588 @default.