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• W2051677861 abstract "DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 81:143-151 (2008) - DOI: https://doi.org/10.3354/dao01945 Loop-mediated isothermal amplification (LAMP) for rapid detection of Renibacterium salmoninarum, the causative agent of bacterial kidney disease Mona Saleh, Hatem Soliman, Mansour El-Matbouli* Clinic for Fish and Reptiles, Faculty of Veterinary Medicine, Kaulbachstr. 37, University of Munich, 80539 Munich, Germany *Corresponding author. Email: el-matbouli@lmu.de ABSTRACT: A loop-mediated isothermal amplification (LAMP) assay was developed for rapid, specific and sensitive detection of Renibacterium salmoninarum in 1 h without thermal cycling. A fragment of R. salmoninarum p57 gene was amplified at 63°C in the presence of Bst polymerase and a specially designed primer mixture. The specificity of the BKD-LAMP assay was demonstrated by the absence of any cross reaction with other bacterial strains, followed by restriction digestion of the amplified products. Detections of BKD-LAMP amplicons by visual inspection, agrose gel electrophoresis, and real-time monitoring using a turbidimeter were equivalently sensitive. The BKD-LAMP assay has the sensitivity of the nested PCR method, and 10 times the sensitivity of one-round PCR assay. The lower detection limit of BKD-LAMP and nested PCR is 1 pg genomic R. salmoninarum DNA, compared to 10 pg genomic R. salmoninarum DNA for one-round PCR assay. In comparison to other available diagnostic methods, the BKD-LAMP assay is rapid, simple, sensitive, specific, and cost effective with a high potential for field application. KEY WORDS: Renibacterium salmoninarum · BKD · LAMP · Diagnosis Full text in pdf format PreviousNextCite this article as: Saleh M, Soliman H, El-Matbouli M (2008) Loop-mediated isothermal amplification (LAMP) for rapid detection of Renibacterium salmoninarum, the causative agent of bacterial kidney disease. Dis Aquat Org 81:143-151. https://doi.org/10.3354/dao01945 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 81, No. 2. Online publication date: August 27, 2008 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2008 Inter-Research." @default.
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• W2051677861 date "2008-08-27" @default.
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• W2051677861 title "Loop-mediated isothermal amplification (LAMP) for rapid detection of Renibacterium salmoninarum, the causative agent of bacterial kidney disease" @default.
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• W2051677861 doi "https://doi.org/10.3354/dao01945" @default.
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