Matches in SemOpenAlex for { <https://semopenalex.org/work/W2051684781> ?p ?o ?g. }
- W2051684781 endingPage "679" @default.
- W2051684781 startingPage "667" @default.
- W2051684781 abstract "Abstract Astrocytes are involved in key physiological brain processes, such as glutamatergic transmission and energy metabolism, often altered in neurodegenerative diseases. Targeted gene expression in astrocytes is needed to assess the contribution of these cells to physiological processes and for the development of new therapeutic strategies. However, most of the viral vectors currently used for gene transfer in the central nervous system (CNS) are highly neurotropic. We used mokola pseudotyping to shift the tropism of lentiviral vectors toward astrocytes and a detargeting strategy with miRNA to eliminate residual expression in neuronal cells. In primary cultures, we showed that incorporating target sequences for the neuron‐specific miR124 effectively abolished transgene expression in neurons post‐transcriptionally. Targeted expression of the LacZ reporter gene in astrocytes was achieved in the hippocampus, striatum, and cerebellum of the adult mouse in vivo . As a proof‐of‐principle, this new lentiviral vector was used to either overexpress or downregulate (RNA interference) the glial glutamate transporter GLAST into striatal astrocytes in vivo . These vectors provide new opportunities for cell type‐specific gene transfer in the CNS. © 2008 Wiley‐Liss, Inc." @default.
- W2051684781 created "2016-06-24" @default.
- W2051684781 creator A5004590713 @default.
- W2051684781 creator A5013839554 @default.
- W2051684781 creator A5025572342 @default.
- W2051684781 creator A5029086103 @default.
- W2051684781 creator A5051377002 @default.
- W2051684781 creator A5065418218 @default.
- W2051684781 creator A5067657844 @default.
- W2051684781 creator A5075424512 @default.
- W2051684781 creator A5075772436 @default.
- W2051684781 creator A5087753311 @default.
- W2051684781 date "2008-10-22" @default.
- W2051684781 modified "2023-10-18" @default.
- W2051684781 title "Engineered lentiviral vector targeting astrocytes<i>In vivo</i>" @default.
- W2051684781 cites W1565258089 @default.
- W2051684781 cites W1904875888 @default.
- W2051684781 cites W1965306643 @default.
- W2051684781 cites W1969305530 @default.
- W2051684781 cites W1970761063 @default.
- W2051684781 cites W1975464681 @default.
- W2051684781 cites W1977301725 @default.
- W2051684781 cites W1978337436 @default.
- W2051684781 cites W1978757999 @default.
- W2051684781 cites W1978836458 @default.
- W2051684781 cites W1983166842 @default.
- W2051684781 cites W1985436254 @default.
- W2051684781 cites W1986195447 @default.
- W2051684781 cites W1993180746 @default.
- W2051684781 cites W1996706043 @default.
- W2051684781 cites W1999829381 @default.
- W2051684781 cites W2003456064 @default.
- W2051684781 cites W2009761723 @default.
- W2051684781 cites W2011072606 @default.
- W2051684781 cites W2017270642 @default.
- W2051684781 cites W2018352999 @default.
- W2051684781 cites W2019028138 @default.
- W2051684781 cites W2022297536 @default.
- W2051684781 cites W2027582763 @default.
- W2051684781 cites W2032730787 @default.
- W2051684781 cites W2034197352 @default.
- W2051684781 cites W2038036971 @default.
- W2051684781 cites W2038253875 @default.
- W2051684781 cites W2042673698 @default.
- W2051684781 cites W2045812968 @default.
- W2051684781 cites W2048371307 @default.
- W2051684781 cites W2054226892 @default.
- W2051684781 cites W2054588014 @default.
- W2051684781 cites W2067102106 @default.
- W2051684781 cites W2068147651 @default.
- W2051684781 cites W2079555292 @default.
- W2051684781 cites W2087128420 @default.
- W2051684781 cites W2094240884 @default.
- W2051684781 cites W2095931805 @default.
- W2051684781 cites W2109971716 @default.
- W2051684781 cites W2114618955 @default.
- W2051684781 cites W2116224426 @default.
- W2051684781 cites W2116641097 @default.
- W2051684781 cites W2120876243 @default.
- W2051684781 cites W2124658196 @default.
- W2051684781 cites W2124940469 @default.
- W2051684781 cites W2126525449 @default.
- W2051684781 cites W2129567809 @default.
- W2051684781 cites W2139871850 @default.
- W2051684781 cites W2141335195 @default.
- W2051684781 cites W2150922307 @default.
- W2051684781 cites W2154909423 @default.
- W2051684781 cites W2163447682 @default.
- W2051684781 cites W2164609794 @default.
- W2051684781 cites W2168458927 @default.
- W2051684781 cites W2171985637 @default.
- W2051684781 cites W2321725396 @default.
- W2051684781 cites W2332224516 @default.
- W2051684781 doi "https://doi.org/10.1002/glia.20795" @default.
- W2051684781 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18942755" @default.
- W2051684781 hasPublicationYear "2008" @default.
- W2051684781 type Work @default.
- W2051684781 sameAs 2051684781 @default.
- W2051684781 citedByCount "130" @default.
- W2051684781 countsByYear W20516847812012 @default.
- W2051684781 countsByYear W20516847812013 @default.
- W2051684781 countsByYear W20516847812014 @default.
- W2051684781 countsByYear W20516847812015 @default.
- W2051684781 countsByYear W20516847812016 @default.
- W2051684781 countsByYear W20516847812017 @default.
- W2051684781 countsByYear W20516847812018 @default.
- W2051684781 countsByYear W20516847812019 @default.
- W2051684781 countsByYear W20516847812020 @default.
- W2051684781 countsByYear W20516847812021 @default.
- W2051684781 countsByYear W20516847812022 @default.
- W2051684781 countsByYear W20516847812023 @default.
- W2051684781 crossrefType "journal-article" @default.
- W2051684781 hasAuthorship W2051684781A5004590713 @default.
- W2051684781 hasAuthorship W2051684781A5013839554 @default.
- W2051684781 hasAuthorship W2051684781A5025572342 @default.
- W2051684781 hasAuthorship W2051684781A5029086103 @default.
- W2051684781 hasAuthorship W2051684781A5051377002 @default.
- W2051684781 hasAuthorship W2051684781A5065418218 @default.