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- W2051750605 abstract "Introduction Thorough understanding of fluid dynamics in root canal irrigation and corresponding antibiofilm capacity will support improved disinfection strategies. This study aimed to develop a standardized, simulated root canal model that allows real-time analysis of fluid/irrigation dynamics and its correlation with biofilm elimination. Methods A maxillary incisor with an instrumented root canal was imaged with micro–computed tomography. The canal volume was reconstructed in 3 dimensions and replicated in soft lithography-based models microfabricated from polyethylene glycol–modified polydimethylsiloxane. Canals were irrigated by using a syringe (SI) and 2 ultrasonic-assisted methods, intermittent (IUAI) and continuous (CUAI). Real-time fluid movement within the apical 3 mm of canals was imaged by using microparticle image velocimetry. In similar models, canals were inoculated with Enterococcus faecalis to grow 3-week-old biofilms. Biofilm reduction by irrigation with SI, CUAI, and IUAI was assessed by using a crystal violet assay and compared with an untreated control. Results SI generated higher velocity and shear stress in the apical 1–2 mm than 0–1 and 2–3 mm. IUAI generated consistently low shear stress in the apical 3 mm. CUAI generated consistently high levels of velocity and shear stress; it was the highest of the groups in the apical 0–1 and 2–3 mm. Biofilm was significantly reduced compared with the control only by CUAI (two-sample permutation test, P = .005). Conclusions CUAI exhibited the highest mechanical effects of fluid flow in the apical 3 mm, which correlated with significant biofilm reduction. The soft lithography-based models provided a novel model/method for study of correlations between fluid dynamics and the antibiofilm capacity of root canal irrigation methods." @default.
- W2051750605 created "2016-06-24" @default.
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- W2051750605 date "2015-06-01" @default.
- W2051750605 modified "2023-10-05" @default.
- W2051750605 title "Fluid Dynamics and Biofilm Removal Generated by Syringe-delivered and 2 Ultrasonic-assisted Irrigation Methods: A Novel Experimental Approach" @default.
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- W2051750605 doi "https://doi.org/10.1016/j.joen.2015.01.027" @default.
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