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• W2051853530 abstract "Primary hepatocytes from wild northern pike Esox lucius were incubated with radiolabelled linolenic acid ([l‐ 14 C]‐18:3(n‐3)) to assess their ability to synthesize docosahexaenoic acid [22:6(n‐3)]. The distribution of radioactivity in lipid classes and hepatocyte polyunsaturated fatty acids (PUFA) was measured over the time‐course of 24h. The majority of radioactivity from [l‐ 14 C]‐18:3(n‐3) was recovered in hepatocyte triacylglycerols (TAG) and phosphatidylcholine (PC). The levels of radioactivity in TAG and in most of phospholipids, including PC, increased significantly over the incubation period. Radioactivity from [1‐ 14 C]‐18:3(n‐3) was recovered in several hepatocyte PUFA, including 22:6(n‐3), and the Δ6 and Δ5‐desaturation products 18:4(n‐3) and 20:5(n‐3). The presence of radioactivity in C 24 (n‐3) PUFA may be evidence that the biosynthesis of 22:6(n‐3) in pike proceeds via a pathway independent of Δ4‐desaturation. Analysis by radio gas chromatography revealed that radiolabelled 24:6(n‐3) was present among the desaturation and elongation products of [l‐ 14 C]‐18:3(n‐3). The results establish that, under the in vitro conditions employed, pike hepatocytes are able to convert linolenic acid to 20:5(n‐3) and 22:6(n‐3)." @default.
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• W2051853530 date "1997-12-01" @default.
• W2051853530 modified "2023-09-26" @default.
• W2051853530 title "The biosynthesis of docosahexaenoic acid [22:6(n-3)] from linolenic acid in primary hepatocytes isolated from wild northern pike" @default.
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• W2051853530 doi "https://doi.org/10.1111/j.1095-8649.1997.tb01136.x" @default.
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